ImmunofluorescenceU87 cells were fixed and permeabilized with 4% Paraformaldehyde and 0.5% Triton
™ X-100. Fixed cells were stained with 5 μg/mL Mouse Anti-LRIG3 Clone: LRG3-3 (
Cat. No. SAB4200368). The antibody was developed using Goat Anti-Mouse IgG, Cy3
™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
ImmunoblottingH1395 cell extract was separated on SDS-PAGE and probed with Mouse Anti-LRIG3 Clone: LRG3-3 (
Cat. No. SAB4200368). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (
Cat. No. A2304) and a chemiluminescent substrate.
Lanes1. 2 μg/mL antibody
2. 2 μg/mL antibody + LRIG3 immunizing peptide (human, 65-82)