ImmunofluorescenceLnCAP cells were fixed and permeabilized with cold methanol followed by acetone. Fixed cells were stained with 2 μg/mL Mouse Anti-LRIG1 Clone: LRIG1-21 (
Cat. No. SAB4200445). The antibody was developed using Goat Anti-Mouse IgG, Cy3
™ conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
ImmunoblottingLnCAP cell extract was separated on SDS-PAGE and probed with Mouse Anti-LRIG1 Clone: LRIG1-21 (
Cat. No. SAB4200445). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (
Cat. No. A2304) and a chemiluminescent substrate.
Lanes1. 2 μg/mL antibody
2. 2 μg/mL antibody + LRIG1 immunizing peptide (human, 62-79)