Monoclonal Anti-NIT2 antibody produced in mouse

Name	Monoclonal Anti-NIT2 antibody produced in mouse
Supplier	Sigma-Aldrich
Catalog	SAB4100052
Prices	$377.00
Sizes	200 µl
Host	Mouse
Clonality	Monoclonal
Isotype	IgG2b
Clone	NIT2-A48
Applications	WB
Species Reactivities	Human
Antigen	NIT2 purified recombinant protein
Description	Mouse Monoclonal
Gene	NIT2
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunofluorescence</B><BR/>A549 cells were fixed and permeabilized with 4% Paraformaldehyde and 0.5% Triton X-100. Fixed cells were stained with Mouse Anti-NIT2 Clone: NIT2-A48 (<B>Cat. No. SAB4100052</B>) diluted to 1:5. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

Immunofluorescence
A549 cells were fixed and permeabilized with 4% Paraformaldehyde and 0.5% Triton X-100. Fixed cells were stained with Mouse Anti-NIT2 Clone: NIT2-A48 (Cat. No. SAB4100052) diluted to 1:5. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells expressing FLAG<SUP>®</SUP>-tagged human NIT2 was separated on SDS-PAGE, probed with Mouse Anti-NIT2 Clone: NIT2-A48 (<B>Cat. No. SAB4100052</B>). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (<B>Cat. No. A2304</B>) and a chemiluminescent substrate.<BR/><B>Lanes</B><BR/>1. Antibody dilution 1:10,000<BR/>2. Negative control<BR/>3. Positive control Anti-FLAG<SUP>®</SUP>

Immunoblotting
Lysate of HEK-293T cells expressing FLAG^®-tagged human NIT2 was separated on SDS-PAGE, probed with Mouse Anti-NIT2 Clone: NIT2-A48 (Cat. No. SAB4100052). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate.
Lanes
1. Antibody dilution 1:10,000
2. Negative control
3. Positive control Anti-FLAG^®

<B>Immunoblotting</B><BR/>A549 cell extract was separated on SDS-PAGE and probed with Mouse Anti-NIT2 Clone: NIT2-A48 (<B>Cat. No. SAB4100048</B>). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (<B>Cat. No. A2304</B>) and a chemiluminescent substrate.<BR/><B>Lanes</B><BR/>1. Antibody dilution 1:50<BR/>2. Antibody dilution 1:100<BR/>3. Negative control

Immunoblotting
A549 cell extract was separated on SDS-PAGE and probed with Mouse Anti-NIT2 Clone: NIT2-A48 (Cat. No. SAB4100048). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (Cat. No. A2304) and a chemiluminescent substrate.
Lanes
1. Antibody dilution 1:50
2. Antibody dilution 1:100
3. Negative control

<B>Flow Cytometry</B><BR/>1×10<SUP>6</SUP> A549 cells were fixed and permeabilized with 2% Paraformaldehyde and 0.1% Triton X-100. Fixed cells were labeled with Mouse Anti-NIT2 Clone: NIT2-A48 (<B>Cat. No. SAB4100052</B>) diluted to 1:10 followed by Rabbit Anti-Mouse IgG, FITC conjugate. Shaded histogram: negative control without first antibody, Gray histogram: antibody specific staining.

Flow Cytometry
1×10⁶ A549 cells were fixed and permeabilized with 2% Paraformaldehyde and 0.1% Triton X-100. Fixed cells were labeled with Mouse Anti-NIT2 Clone: NIT2-A48 (Cat. No. SAB4100052) diluted to 1:10 followed by Rabbit Anti-Mouse IgG, FITC conjugate. Shaded histogram: negative control without first antibody, Gray histogram: antibody specific staining.