NIT2

Gene Symbol NIT2
Entrez Gene 56954
Alt Symbol HEL-S-8a
Species Human
Gene Type protein-coding
Description nitrilase family, member 2
Other Description Nit protein 2|epididymis secretory sperm binding protein Li 8a|nitrilase homolog 2|omega-amidase NIT2
Swissprots Q9NQR4 B2R9A3 Q8WUF0 D3DN47
Accessions EAW79824 EAW79825 Q9NQR4 AF260334 AAG44665 AF284574 AAF87103 AK225979 AK294010 BAG57372 AK295835 BAH12195 AK313704 BAG36450 BC020620 AAH20620 BC107890 AAI07891 BM748852 DB466042 DQ893135 ABM84061 DQ896416 ABM87415 FJ224326 ACI46018 NM_020202 NP_064587
Function Has a omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha- ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. Overexpression decreases the colony-forming capacity of cultured cells by arresting cells in the G2 phase of the cell cycle. {ECO:0000269|PubMed:17488281, ECO:0000269|PubMed:19595734}.
Subcellular Location Cytoplasm {ECO:0000269|PubMed:17488281}.
Tissue Specificity Detected in fetal brain (at protein level). Ubiquitous. Detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, prostate, spleen, thymus, prostate, testis, ovary, small intestine and colon. {ECO:0000269|PubMed:14528910, ECO:0000269|PubMed:17488281}.
Top Pathways Alanine, aspartate and glutamate metabolism

NIT2 (C-13) - sc-103073 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Anti-NIT2 antibody - ab183074 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-NIT2 antibody (ab183074) at 1/950 dilutionLane 1 : mouse liver tissue lysateLane 2 : mouse kidney tissue lysateLane 3 : RAW264.7 cell lysateLysates/proteins at 40 µg per lane.SecondaryGoat anti Rabbit IgG - H&L (HRP) at 1/10000 dilutiondeveloped using the ECL technique

Anti-NIT2 antibody - ab203245 from Abcam

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Mouse embryo tissue labeling NIT2 with ab203245 at 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Anti-NIT2 antibody [1A6] - ab118153 from Abcam

Prices $370.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1A6
All lanes : Anti-NIT2 antibody [1A6] (ab118153) at 1/1000 dilutionLane 1 : HEK293T cell lysate transfected with pCMV6-ENTRY Control cDNALane 2 : HEK293T cell lysate transfected with pCMV6-ENTRY NIT2 cDNALysates/proteins at 5 µg per lane.

Anti-NIT2 Antibody (clone 1A6) - LS-C115174 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 1A6
IHC of paraffin-embedded Human liver tissue using anti-NIT2 mouse monoclonal antibody. (Dilution 1:50).

Anti-NIT2 Antibody (clone 5A3) - LS-C115160 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 5A3
IHC of paraffin-embedded liver tissue using anti-NIT2 mouse monoclonal antibody. (Dilution 1:50).

Anti-NIT2 Antibody (clone 3F3) - LS-C115138 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3F3
IHC of paraffin-embedded liver tissue using anti-NIT2 mouse monoclonal antibody. (Dilution 1:50).

Anti-NIT2 Antibody (clone 6H7) - LS-C115122 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 6H7
IHC of paraffin-embedded Carcinoma of prostate tissue using anti-NIT2 mouse monoclonal antibody. (Dilution 1:50).

Anti-NIT2 Antibody (aa72-121) - LS-C110873 from LifeSpan Bioscience

Host Rabbit
Clonality Polyclonal
NIT2 antibody LS-C110873 Western blot of Fetal Liver lysate.

NIT2 Antibody (2B9) - NBP2-02486 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 2B9

Anti-NIT2 antibody produced in rabbit - HPA036999 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-NIT2: <B>Cat. No. HPA036999</B>: Immunohistochemical staining of human liver shows strong cytoplasmic positivity in hepatocytes.

Monoclonal Anti-NIT2 antibody produced in mouse - SAB4100052 from Sigma-Aldrich

Prices $377.00
Sizes 200 µl
Host Mouse
Clonality Monoclonal
Clone NIT2-A48
<B>Immunofluorescence</B><BR/>A549 cells were fixed and permeabilized with 4% Paraformaldehyde and 0.5% Triton X-100. Fixed cells were stained with Mouse Anti-NIT2 Clone: NIT2-A48 (<B>Cat. No. SAB4100052</B>) diluted to 1:5. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

Monoclonal Anti-NIT2 antibody produced in mouse - SAB4100070 from Sigma-Aldrich

Prices $358.00
Sizes 200 µl
Host Mouse
Clonality Monoclonal
Clone NIT2-B48
<B>Immunofluorescence</B><BR/>A549 cells were fixed and permeabilized with 4% Paraformaldehyde and 0.5% Triton X-100. Fixed cells were stained with Mouse Anti-NIT2 Clone: NIT2-B48 (<B>Cat. No. SAB4100070</B>) diluted to 1:50. The antibody was developed using Goat Anti-Mouse IgG, Atto 488 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.

Anti-NIT2 (56-70) antibody produced in rabbit - N4664 from Sigma-Aldrich

Prices $328.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunofluorescence</B><BR/>Anti-NIT2 (56-70): <B>Cat. No. N4664</B>: Immunofluorescence of HUVEC cells using NIT2 (56-70) (RB),<B>Cat. No. N4664</B> (red) at a 1:50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Anti-NIT2 (132-145) antibody produced in rabbit - N4789 from Sigma-Aldrich

Prices $328.50
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunofluorescence</B><BR/>Anti-NIT2 (132-145): <B>Cat. No. N4789</B>: Immunofluorescence of HUVEC cells using NIT2 (132-145) (RB),<B>Cat. No. N4789</B> (red) at a 1:200 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

NIT2 antibody - middle region (ARP46292_P050) - ARP46292_P050 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

NIT2 antibody [1A6] - GTX84035 from GeneTex

Prices $329.00
Sizes 100 μl
Host Mouse
Clonality Monoclonal
Clone 1A6
Anti-NIT2 antibody [1A6] used in IHC (Formalin-fixed paraffin-embedded sections). GTX84035

NIT2 antibody [2B9] - GTX84036 from GeneTex

Prices $329.00
Sizes 100 μl
Host Mouse
Clonality Monoclonal
Clone 2B9
Anti-NIT2 antibody [2B9] used in Flow cytometry/FACS. GTX84036

NIT2 antibody [3D5] - GTX84039 from GeneTex

Prices $329.00
Sizes 100 μl
Host Mouse
Clonality Monoclonal
Clone 3D5
Anti-NIT2 antibody [3D5] used in Flow cytometry/FACS. GTX84039

NIT2 antibody [3F3] - GTX84038 from GeneTex

Prices $329.00
Sizes 100 μl
Host Mouse
Clonality Monoclonal
Clone 3F3
Anti-NIT2 antibody [3F3] used in ICC/IF. GTX84038

NIT2 antibody [5A3] - GTX84037 from GeneTex

Prices $329.00
Sizes 100 μl
Host Mouse
Clonality Monoclonal
Clone 5A3
Anti-NIT2 antibody [5A3] used in IHC (Formalin-fixed paraffin-embedded sections). GTX84037

NIT2 antibody [6H7] - GTX84040 from GeneTex

Prices $329.00
Sizes 100 μl
Host Mouse
Clonality Monoclonal
Clone 6H7
Anti-NIT2 antibody [6H7] used in IHC (Formalin-fixed paraffin-embedded sections). GTX84040

NIT2 antibody - 10R-4990 from Fitzgerald

Prices $395.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 6H7

NIT2 antibody - 10R-4995 from Fitzgerald

Prices $395.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 5A3

NIT2 antibody - 10R-4992 from Fitzgerald

Prices $395.00
Sizes 100 µl
Host Mouse
Clonality Monoclonal
Clone 3F3
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