Anti-PIP5K1B (491-505) antibody produced in rabbit

Name	Anti-PIP5K1B (491-505) antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	I6660
Prices	$328.50
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal
Applications	WB
Species Reactivities	Human
Antigen	synthetic peptide corresponding to amino acids 491-505 of human PIP5K1B
Description	Rabbit Polyclonal
Gene	PIP5K1B
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunofluorescence</B><BR/>Anti-PIP5K1B (491-505) (RB): <B>Cat. No. I6660</B>: Immunofluorescence of HUVEC cells using PIP5K1B (491-505) (RB), <B>Cat. No. I6660</B> (red) at a 1:50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue). Yale HTCB IF procedure used.<BR/>Images have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Immunofluorescence
Anti-PIP5K1B (491-505) (RB): Cat. No. I6660: Immunofluorescence of HUVEC cells using PIP5K1B (491-505) (RB), Cat. No. I6660 (red) at a 1:50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue). Yale HTCB IF procedure used.
Images have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human PIP5K1B was separated on SDS-PAGE, blotted with Anti-PIP5K1B (491-505) (<B>Cat. No. I6660</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate.<BR/><B>Lanes</B><BR/>1. Antibody dilution 1:500<BR/>2. Antibody dilution 1:500 + PIP5K1B immunizing peptide (human, 491-505)<BR/>3. Antibody dilution 1:1,000

Immunoblotting
Lysate of HEK-293T cells overexpressing human PIP5K1B was separated on SDS-PAGE, blotted with Anti-PIP5K1B (491-505) (Cat. No. I6660) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. Antibody dilution 1:500
2. Antibody dilution 1:500 + PIP5K1B immunizing peptide (human, 491-505)
3. Antibody dilution 1:1,000

<B>Chart</B><BR/>Anti-PIP5K1B (491-505) (RB): <B>Cat. No. I6660</B>: Intensity analysis of Anti-PIP5K1B (491-505) (RB) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.

Chart
Anti-PIP5K1B (491-505) (RB): Cat. No. I6660: Intensity analysis of Anti-PIP5K1B (491-505) (RB) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.