Anti-VPS4B antibody produced in rabbit

Name	Anti-VPS4B antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	SAB4200023
Prices	$61.10, $360.00
Sizes	25 µl, 200 µl
Host	Rabbit
Clonality	Polyclonal
Applications	IP WB
Species Reactivities	Human, Mouse
Antigen	synthetic peptide corresponding to amino acids 81-93 of human VPS4B, conjugated to KLH
Description	Rabbit Polyclonal
Gene	VPS4B
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunoprecipitation</B><BR/>Rabbit Anti-VPS4B (<B>Cat. No. SAB4200023</B>), was used to immunoprecipitate VPS4B from 3T3 whole cell extract. Detection antibody: Rabbit Anti-VPS4 (<B>Cat. No.SAB4200025</B>) followed by Monoclonal Anti-Rabbit IgG native-peroxidase (<B>Cat. No. R3155</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. Positive control: 3T3 whole cell extract<BR/>2. 20 μg antibody <BR/>3. 5 μg antibody <BR/>4. Negative control: 5 μg nonrelevant antibody

Immunoprecipitation
Rabbit Anti-VPS4B (Cat. No. SAB4200023), was used to immunoprecipitate VPS4B from 3T3 whole cell extract. Detection antibody: Rabbit Anti-VPS4 (Cat. No.SAB4200025) followed by Monoclonal Anti-Rabbit IgG native-peroxidase (Cat. No. R3155) and a chemiluminescent substrate.
Lanes
1. Positive control: 3T3 whole cell extract
2. 20 μg antibody
3. 5 μg antibody
4. Negative control: 5 μg nonrelevant antibody

<B>Immunoblotting</B><BR/>Whole extract of G361 cells was separated on SDS-PAGE and probed with Rabbit Anti-VPS4B (<B>Cat. No. SAB4200023</B>). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. 2 μg/mL antibody<BR/>2. 2 μg/mL antibody + VPS4B immunizing peptide (human, 81-93) <BR/>3. 1 μg/mL antibody

Immunoblotting
Whole extract of G361 cells was separated on SDS-PAGE and probed with Rabbit Anti-VPS4B (Cat. No. SAB4200023). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. 2 μg/mL antibody
2. 2 μg/mL antibody + VPS4B immunizing peptide (human, 81-93)
3. 1 μg/mL antibody