VPS4B

Gene Symbol VPS4B
Entrez Gene 9525
Alt Symbol MIG1, SKD1, SKD1B, VPS4-2
Species Human
Gene Type protein-coding
Description vacuolar protein sorting 4 homolog B (S. cerevisiae)
Other Description cell migration-inducing 1|cell migration-inducing gene 1 protein|suppressor of K(+) transport growth defect 1|suppressor of K+ transport defect 1|vacuolar protein sorting 4B|vacuolar protein sorting-associated protein 4B
Swissprots Q9GZS7 Q69HW4 O75351
Accessions AAG01471 EAW63143 EAW63144 O75351 AF038960 AAC39874 AF195514 AAG33022 AK290932 BAF83621 AK291253 BAF83942 AK308892 AK309322 AK311041 AY232629 AAP59551 BC009692 AAH09692 BC039574 AAH39574 DA306069 EU831666 ACE87266 EU831748 ACE86582 HQ448561 ADQ33040 XM_006722582 XP_006722645 XM_011526268 XP_011524570 XR_935266 XR_935267 NM_004869 NP_004860
Function Involved in late steps of the endosomal multivesicular bodies (MVB) pathway. Recognizes membrane-associated ESCRT-III assemblies and catalyzes their disassembly, possibly in combination with membrane fission. Redistributes the ESCRT-III components to the cytoplasm for further rounds of MVB sorting. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. In conjunction with the ESCRT machinery also appears to function in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and enveloped virus budding (HIV-1 and other lentiviruses). {ECO:0000269|PubMed:11563910, ECO:0000269|PubMed:14505570, ECO:0000269|PubMed:18687924}.
Subcellular Location Prevacuolar compartment membrane; Peripheral membrane protein. Late endosome membrane {ECO:0000305}; Peripheral membrane protein {ECO:0000305}. Note=Membrane-associated in the prevacuolar endosomal compartment. Localized in HIV-1 particles purified from acutely infected cells.
Tissue Specificity Ubiquitously expressed. {ECO:0000269|PubMed:11563910}.
Top Pathways Endocytosis

VPS4 (A-11) - sc-393428 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
VPS4 (A-11): sc-393428. Western blot analysis of VPS4A expression in non-transfected 293T: sc-117752 (A), human VPS4A transfected 293T: sc-178127 (B) and MCF7 (C) whole cell lysates.

VPS4B (A-11) - sc-377162 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
VPS4B (A-11): sc-377162. Western blot analysis of VPS4B expression in MIA PaCa-2 (A), K-562 (B), Hep G2 (C) and MCF7 (D) whole cell lysates.

VPS4B (N-12) - sc-21464 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

VPS4B (C-13) - sc-21466 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
VPS4B (C-13): sc-21466. Immunoperoxidase staining of formalin fixed, paraffin-embedded human vagina tissue showing cytoplasmic staining of squamous epithelial cells.

Anti-VPS4B antibody - ab86303 from Abcam

Prices $370.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-VPS4B antibody (ab86303) at 0.04 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : HeLa whole cell lysate at 5 µgLane 4 : 293T whole cell lysate at 50 µgdeveloped using the ECL technique

Anti-VPS4B antibody - ab81416 from Abcam

Prices $370.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-VPS4B antibody (ab81416) at 1 µg/mlLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell LysateLane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell LysateLane 5 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate Lane 6 : Saos 2 (Human epithelial-like osteosarcoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

Anti-VPS4B antibody [EPR9225] - ab137027 from Abcam

Prices $357.00
Sizes 100 µl
Host Rabbit
Clonality Monoclonal
Clone EPR9225
All lanes : Anti-VPS4B antibody [EPR9225] (ab137027) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : 293T cell lysateLane 3 : Jurkat cell lysateLane 4 : HepG2 cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-SKD1 / VPS4B Antibody (aa304-332, APC) - LS-C242616 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SKD1 / VPS4B Antibody (aa304-332, AP) - LS-C242617 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SKD1 / VPS4B Antibody (aa304-332, Biotin) - LS-C242619 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SKD1 / VPS4B Antibody (aa304-332, FITC) - LS-C242620 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SKD1 / VPS4B Antibody (aa304-332, PE) - LS-C242622 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SKD1 / VPS4B Antibody (aa70-119) - LS-C145658 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
VPS4B antibody LS-C145658 Western blot of 3 Cell lysate. Antibody concentration 1 ug/ml.

Anti-SKD1 / VPS4B Antibody (aa300-350) - LS-C288834 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-SKD1 / VPS4B Antibody (aa350-400) - LS-C288837 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-SKD1 / VPS4B Antibody (aa303-332) - LS-C326295 from LifeSpan Bioscience

Prices $345.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-SKD1 / VPS4B Antibody (aa304-332) - LS-C160541 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
VPS4B Antibody western blot of Jurkat cell line lysates (35 ug/lane). The VPS4B antibody detected the VPS4B protein (arrow).

Anti-SKD1 / VPS4B Antibody (aa304-332, Azide-free, HRP) - LS-C247228 from LifeSpan Bioscience

Prices $545.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

VPS4B Antibody - NBP1-19119 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

VPS4B Antibody - NBP1-19118 from Novus Biologicals

Prices $359.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

VPS4B Antibody - NBP2-20881 from Novus Biologicals

Prices $329.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: VPS4B Antibody [NBP2-20881] - Sample (30 ug of whole cell lysate) A: 293T 10% SDS PAGE gel, diluted at 1:3000.

VPS4B Antibody - H00009525-A01 from Novus Biologicals

Prices $269.00
Sizes 50 µl
Host Mouse
Clonality Polyclonal
Western Blot: VPS4B Antibody [H00009525-A01] - Analysis of VPS4B expression in Y-79 (Cat # L042V1).

Anti-VPS4B antibody produced in rabbit - SAB2107645 from Sigma-Aldrich

Prices $341.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-VPS4B: <B>Cat. No. SAB2107645</B>: Western blot analysis of VPS4B in OVCAR-3 with VPS4B (vacuolar protein sorting 4 homolog B, S. cerevisiae) antibody, against the N terminal of VPS4B (50 μg) antibody at 1.0 μg/mL.

Anti-VPS4B antibody produced in rabbit - SAB4200023 from Sigma-Aldrich

Prices $61.10, $360.00
Sizes 25 µl, 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoprecipitation</B><BR/>Rabbit Anti-VPS4B (<B>Cat. No. SAB4200023</B>), was used to immunoprecipitate VPS4B from 3T3 whole cell extract. Detection antibody: Rabbit Anti-VPS4 (<B>Cat. No.SAB4200025</B>) followed by Monoclonal Anti-Rabbit IgG native-peroxidase (<B>Cat. No. R3155</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. Positive control: 3T3 whole cell extract<BR/>2. 20 μg antibody <BR/>3. 5 μg antibody <BR/>4. Negative control: 5 μg nonrelevant antibody

ANTI-VPS4B (C-TERM) antibody produced in rabbit - SAB1304819 from Sigma-Aldrich

Prices $326.50
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-VPS4B (C-term): <B>Cat. No. SAB1304819</B>: VPS4B Antibody (C-term) western blot analysis in Jurkat cell line lysates (35 μg/lane).
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