ImmunofluorescenceNRK cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.4% saponin. Fixed cells were stained with 10 μg/mL Rabbit Anti-Rabex-5 (N-terminal) (
Cat. No. R5405). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate.
ImmunoblottingWhole extract of Mouse Brain was separated on SDS-PAGE and probed with Rabbit Anti-Rabex-5 (N-terminal) (
Cat. No. R5405). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (
Cat. No. A0545) and a chemiluminescent substrate.
Lanes1. 2 μg/mL antibody
2. 2 μg/mL antibody + Rabex-5 immunizing peptide (human, 49-60)
3. 5 μg/mL antibody