Immunoblotting Lysates of the indicated cells were separated on SDS-PAGE and probed with 2 μg/mL of Rabbit Anti-Importin 9 (C-terminal) (
Cat. No. SAB4200154). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (
Cat. No. A0545) and a chemiluminescence substrate.
Lanes1. SK-N-MC
2. HeLa
3. CHO
4. K-562
5. HEK-293T
ImmunoprecipitationRabbit Anti-Importin 9 (C-Terminal) (
Cat. No. SAB4200154) was used to immunoprecipitate human Importin 9 from lysate of HEK-293T cells. Detection antibody: Anti-Importin 9 (C-Terminal)
Lanes1. IP antibody: 5 μg antibody (
Cat. No. SAB4200154)
2. IP antibody: 10 μg antibody
3. Negative control: IP antibody without cell lysate
4. Negative control: Without IP antibody
5. Immunoblotting positive control: total HEK-293T cell lysate