Anti-DPT (161-175) antibody produced in rabbit

• Supplier: Sigma-Aldrich
• Catalog: D8945
• Prices: $328.50
• Sizes: 200 µl
• Host: Rabbit
• Clonality: Polyclonal
• Applications: WB
• Species Reactivities: Human
• Antigen: synthetic peptide corresponding to amino acids 161-175 of human DPT
• Description: Rabbit Polyclonal
• Gene: DPT
• Conjugate: unconjugated

Product images

Immunofluorescence
Anti-DPT (161-175), Cat. No. D8945: Immunofluorescence of HUVEC cells using Anti-DPT (161-175), D8945 (red), taken at 40× magnification and nuclear staining with Hoescht 33342 (blue). Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Immunoblotting
Lysate of HEK-293T cells overexpressing human DPT was separated on SDS-PAGE, blotted with Anti-DPT (161-175) (Cat. No. D8945) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. Antibody dilution 1:500
2. Antibody dilution 1:500 + DPT immunizing peptide (human, 161-175)

Immunofluorescence Graph
Anti-DPT (161-175), Cat. No. D8945: Intensity analysis of Anti-DPT (161-175) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different Antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.

Product References

Dermatopontin expression is decreased in hypertrophic scar and systemic sclerosis - Dermatopontin expression is decreased in hypertrophic scar and systemic sclerosis

Kuroda K, Okamoto O, Shinkai H. J Invest Dermatol. 1999 May;112(5):706-10.

Complementary DNA sequence and chromosomal mapping of a human - Complementary DNA sequence and chromosomal mapping of a human

Superti-Furga A, Rocchi M, Schafer BW, Gitzelmann R. Genomics. 1993 Aug;17(2):463-7.