Anti-AtMPK4 antibody produced in rabbit

Name	Anti-AtMPK4 antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	A6979
Prices	$477.50
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal
Applications	WB
Species Reactivities	Arabidopsis thaliana
Antigen	synthetic peptide corresponding to the C-terminus of Arabidopsis thaliana MPK4 (amino acids 365-376)
Description	Rabbit Polyclonal
Gene	MPK4
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunoblotting</B><BR/>Arabidopsis thaliana whole leaf extracts (Lanes A, F), seedlings (Lanes B, E), flowers (Lane C) and roots (Lane D) were separated on SDS-PAGE and blotted with Rabbit Anti-AtMPK4 (<B>Cat. No. A6979</B>). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (<B>Cat. No. A0545</B>) and a chemiluminescent substrate.<BR/>Antibody concentration 1.0 μg/mL<BR/>Specificity is demonstrated by inhibition of antibody staining of whole leaf extract with the AtMPK4 immunizing peptide (20 μg/mL, Lane F).

Immunoblotting
Arabidopsis thaliana whole leaf extracts (Lanes A, F), seedlings (Lanes B, E), flowers (Lane C) and roots (Lane D) were separated on SDS-PAGE and blotted with Rabbit Anti-AtMPK4 (Cat. No. A6979). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescent substrate.
Antibody concentration 1.0 μg/mL
Specificity is demonstrated by inhibition of antibody staining of whole leaf extract with the AtMPK4 immunizing peptide (20 μg/mL, Lane F).

<B>Immunoblotting</B><BR/><I>Arabidopsis thaliana</I> whole leaf extract was separated by SDS-PAGE and blotted with: (Lane A) Rabbit Anti-AtMPK3 (0.5 μg/mL) (<B>Cat. No. M8318</B>); (Lane B) Rabbit Anti-AtMPK4 (0.5 μg/mL), (<B>Cat. No. A6979</B>); (Lane C) Rabbit Anti-AtMPK6 (0.25 μg/mL), (<B>Cat. No. A7104</B>). The antibodies were developed with Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate.

Immunoblotting
Arabidopsis thaliana whole leaf extract was separated by SDS-PAGE and blotted with: (Lane A) Rabbit Anti-AtMPK3 (0.5 μg/mL) (Cat. No. M8318); (Lane B) Rabbit Anti-AtMPK4 (0.5 μg/mL), (Cat. No. A6979); (Lane C) Rabbit Anti-AtMPK6 (0.25 μg/mL), (Cat. No. A7104). The antibodies were developed with Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.

Product References

MAP kinase signalling cascade in Arabidopsis innate immunity. - MAP kinase signalling cascade in Arabidopsis innate immunity.

Asai T, Tena G, Plotnikova J, Willmann MR, Chiu WL, Gomez-Gomez L, Boller T, Ausubel FM, Sheen J. Nature. 2002 Feb 28;415(6875):977-83.

Mitogen-activated protein kinase cascades in plants: a new nomenclature. - Mitogen-activated protein kinase cascades in plants: a new nomenclature.

. Trends Plant Sci. 2002 Jul;7(7):301-8.

Phytosphingosine-phosphate is a signal for AtMPK6 activation and Arabidopsis - Phytosphingosine-phosphate is a signal for AtMPK6 activation and Arabidopsis

Dutilleul C, Benhassaine-Kesri G, Demandre C, Reze N, Launay A, Pelletier S, Renou JP, Zachowski A, Baudouin E, Guillas I. New Phytol. 2012 Apr;194(1):181-91.

ATMPKs: a gene family of plant MAP kinases in Arabidopsis thaliana. - ATMPKs: a gene family of plant MAP kinases in Arabidopsis thaliana.

Mizoguchi T, Hayashida N, Yamaguchi-Shinozaki K, Kamada H, Shinozaki K. FEBS Lett. 1993 Dec 28;336(3):440-4.