ImmunofluorescenceAnti-ARIH1 (1-14):
Cat. No. A5108: Immunofluorescence of HUVEC cells using ARIH1 (1-14) (RB),
Cat. No. A5108 (red) at a 1:100 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com
ImmunoblottingLysate of HEK-293T cells overexpressing human ARIH1 was separated on SDS-PAGE, blotted with Anti-ARIH1 (1-14) (
Cat. No. A5108) and developed using Goat Anti-Rabbit IgG-Peroxidase (
Cat. No. A0545) and a chemiluminescent substrate.
Lanes1. Antibody dilution 1:500
2. Antibody dilution 1:500 + ARIH1 immunizing peptide (human, 1-14)
ChartAnti-ARIH1 (1-14):
Cat. No. A5108: Intensity analysis of Anti-ARIH1 (1-14) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.