ARIH1

Gene Symbol ARIH1
Entrez Gene 25820
Alt Symbol ARI, HARI, HHARI, UBCH7BP
Species Human
Gene Type protein-coding
Description ariadne RBR E3 ubiquitin protein ligase 1
Other Description ARI-1|E3 ubiquitin-protein ligase ARIH1|H7-AP2|MOP-6|ariadne homolog, ubiquitin-conjugating enzyme E2 binding protein, 1|ariadne, Drosophila, homolog of|monocyte protein 6|protein ariadne-1 homolog|ubcH7-binding protein|ubcM4-interacting protein|ubiquitin-conjugating enzyme E2-binding protein 1
Swissprots Q9UP39 Q9H3T6 O76026 B2R6U3 Q9UEN0 Q9Y4X5
Accessions CAH05382 EAW77907 EAW77908 Q9Y4X5 AB014774 BAB19786 AB209432 BAD92669 AF072832 AAD28088 AJ009771 CAA08817 AJ130976 CAA10274 AJ243190 CAB45870 AK000336 AK312715 BAG35590 AL117650 BC051877 AAH51877 BX641117 XM_011521438 XP_011519740 XM_011521439 XP_011519741 NM_005744 NP_005735
Function E3 ubiquitin-protein ligase, which catalyzes polyubiquitination of target proteins together with ubiquitin- conjugating enzyme E2 UBE2L3. May play a role in protein translation by mediating polyubiquitination of EIF4E2, leading to its subsequent degradation. Acts as the ligase involved in ISGylation of EIF4E2. {ECO:0000269|PubMed:14623119, ECO:0000269|PubMed:15236971, ECO:0000269|PubMed:17289916, ECO:0000269|PubMed:21532592}.
Subcellular Location Cytoplasm {ECO:0000269|PubMed:11278816}. Note=Mainly cytoplasmic.
Tissue Specificity Widely expressed. {ECO:0000269|PubMed:10521492}.

ARIH1 (B-2) - sc-390763 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
ARIH1 (B-2): sc-390763. Western blot analysis of ARIH1 expression in Jurkat whole cell lysate.

ARIH1 (C-13) - sc-131868 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
ARIH1 (C-13): sc-131868. Western blot analysis of ARIH1 expression in non-transfected 293T: sc-117752 (A), human ARIH1 transfected 293T: sc-116302 (B), Jurkat (C) and U-937 (D) whole cell lysates.

ARIH1 (Q-20) - sc-101889 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
ARIH1 (Q-20): sc-101889. Western blot analysis of ARIH1 expression in Jurkat whole cell lysate.

Anti-ARIH1 antibody - ab3891 from Abcam

Prices $370.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
All lanes : Anti-ARIH1 antibody (ab3891) at 1/1500 dilutionLane 1 : NIH3T3 whole cell lysate at 30 µgLane 2 : NIH3T3 whole cell lysate at 30 µgLane 3 : Partially purified NIH3T3 protein mixture at 10 µgSecondaryAlkaline Phosphatase-conjugated anti-goat IgG polyclonal at 1/15000 dilutionPerformed under reducing conditions.Observed band size : 65 kDa (why is the actual band size different from the predicted?)Additional bands at : 45 kDa (possible non-specific binding),50 kDa (possible non-specific binding).Exposure time : 15 minutesThis image is courtesy of an Abreview submitted by T LawsonLanes 1 and 2 - 12% SDS-PAGE.Lane 3 - 4-15% SDS-PAGE.Lanes 1 and 3 - developed with colorimetric substrate.Lane 2 - developed with ECF+.See Abreview

Anti-ARIH1 Antibody (aa513-526) IHC-plus™ - LS-B2620 from LifeSpan Bioscience

Prices $395.00
Sizes 50 µg
Host Goat
Clonality Polyclonal
Anti-ARIH1 antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2620 concentration 5 ug/ml.

Anti-ARIH1 Antibody (C-Terminus) - LS-C31922 from LifeSpan Bioscience

Prices $325.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Jurkat Cell Lysate

ARIH1 Antibody - NBP1-55039 from Novus Biologicals

Prices $139.00, $299.00
Sizes 20 µl, 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: ARIH1 Antibody [NBP1-55039] - Jurkat cell lysate, concentration 1.25ug/ml.

ARIH1 Antibody - NBP1-88984 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: ARIH1 Antibody [NBP1-88984] - Analysis in control (vector only transfected HEK293T lysate) and LY417059 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).

ARIH1 Antibody - NB100-1162 from Novus Biologicals

Prices $299.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
Western Blot: ARIH1 Antibody [NB100-1162] - (0.5ug/ml) of U937 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Anti-ARIH1 (1-14) antibody produced in rabbit - A5108 from Sigma-Aldrich

Prices $342.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunofluorescence</B><BR/>Anti-ARIH1 (1-14): <B>Cat. No. A5108</B>: Immunofluorescence of HUVEC cells using ARIH1 (1-14) (RB),<B>Cat. No. A5108</B> (red) at a 1:100 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Anti-ARIH1/HHARI antibody produced in goat - SAB2500105 from Sigma-Aldrich

Prices $376.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-ARIH1 / HHARI: <B>Cat. No. SAB2500105</B>: staining (0.5 μg/mL) of U937 lysate (RIPA buffer, 30 μg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Anti-ARIH1 antibody produced in rabbit - HPA003295 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-ARIH1: <B>Cat. No. HPA003295</B>: Immunoperoxidase staining of formalin-fixed, paraffin-embedded human small intestine shows cytoplasmic positivity in glandular cells.

Anti-ARIH1 antibody produced in rabbit - AV43054 from Sigma-Aldrich

Prices $267.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Cell Type: Jurkat (<B>Cat. No. AV43054</B>).<BR/><B>Lanes</B><BR/>1. Antibody dilution (concentration) 1.25 μg/mL in cell type Jurkat

ARIH1 antibody - C-terminal region (ARP43054_T100) - ARP43054_T100 from Aviva Systems Biology

Prices $229.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

ARIH1--HHARI-Antibody - AF1100a from Abgent, a WuXi AppTec company

Prices $335.00
Sizes 100 µg
Host Goat
Clonality Polyclonal
AF1100a staining (0.5 µg/ml) of U937 lysate (RIPA buffer, 30 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

ARIH1 / HHARI antibody, C-term - GTX89873 from GeneTex

Prices $319.00
Sizes 100 μg
Host Goat
Clonality Polyclonal
Anti-ARIH1 / HHARI antibody, C-term used in Western blot. GTX89873

ARIH1 antibody - GTX23891 from GeneTex

Prices $319.00
Sizes 50 μg
Host Goat
Clonality Polyclonal
Anti-ARIH1 antibody used in Western blot. GTX23891

ARIH1 antibody [C3], C-term - GTX106385 from GeneTex

Host Rabbit
Clonality Polyclonal
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