Anti-EXOSC4 antibody produced in rabbit

Name	Anti-EXOSC4 antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	SAB4200397
Prices	$343.50
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal
Applications	IP ICC/IF WB
Species Reactivities	Human, Mouse
Antigen	peptide corresponding to the N-terminal region of human EXCSC4, conjugated to KLH
Description	Rabbit Polyclonal
Gene	EXOSC4
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunofluorescence</B><BR/>HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton<SUP>™</SUP> X-100. Fixed cells were stained with 5 μg/mL Rabbit Anti-EXOSC4 (<B>Cat. No. SAB4200397</B>). The antibody was developed using Goat Anti-Rabbit IgG, Atto-488 conjugate (<B>Cat. No. 18772</B>).

Immunofluorescence
HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton^™ X-100. Fixed cells were stained with 5 μg/mL Rabbit Anti-EXOSC4 (Cat. No. SAB4200397). The antibody was developed using Goat Anti-Rabbit IgG, Atto-488 conjugate (Cat. No. 18772).

<B>Immunoblotting</B><BR/>Cell line lysates were separated on SDS-PAGE and probed with 4 μg/mL Rabbit Anti-EXOSC4 (<B>Cat. No. SAB4200397</B>). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate.<BR/><B>Lanes</B><BR/>1. HepG2<BR/>2. MCF7<BR/>3. HeLa<BR/>4. MBDK<BR/>5. MDCK<BR/>6. NRK<BR/>7. NIH-3T3<BR/>8. Hepa 1-6<BR/>9. HEK-293T

Immunoblotting
Cell line lysates were separated on SDS-PAGE and probed with 4 μg/mL Rabbit Anti-EXOSC4 (Cat. No. SAB4200397). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. HepG2
2. MCF7
3. HeLa
4. MBDK
5. MDCK
6. NRK
7. NIH-3T3
8. Hepa 1-6
9. HEK-293T

<B>Immunoprecipitation</B><BR/>Rabbit Anti-EXOSC4 (<B>Cat. No. SAB4200397</B>) was used to immunoprecipitate EXOSC4 from HeLa whole cell extract. Detection antibody: Rabbit Anti-EXOSC4 (<B>Cat. No. SAB4200397</B>).<BR/><B>Lanes</B><BR/>1. Positive control: HeLa whole cell extract<BR/>2. 5 μg antibody<BR/>3. 2.5 μg antibody<BR/>4. 1.25 μg antibody<BR/>5. Negative control: 5 μg non-relevant antibody

Immunoprecipitation
Rabbit Anti-EXOSC4 (Cat. No. SAB4200397) was used to immunoprecipitate EXOSC4 from HeLa whole cell extract. Detection antibody: Rabbit Anti-EXOSC4 (Cat. No. SAB4200397).
Lanes
1. Positive control: HeLa whole cell extract
2. 5 μg antibody
3. 2.5 μg antibody
4. 1.25 μg antibody
5. Negative control: 5 μg non-relevant antibody