Anti-VAC14 antibody produced in rabbit

Name	Anti-VAC14 antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	SAB4200074
Prices	$61.10, $360.00
Sizes	25 µl, 200 µl
Host	Rabbit
Clonality	Polyclonal
Applications	IP ICC/IF WB
Species Reactivities	Human
Antigen	synthetic peptide corresponding to amino acids 341-359 of human VAC14, conjugated to KLH
Description	Rabbit Polyclonal
Gene	VAC14
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunofluorescence</B><BR/>Cytoplasmic and nuclear localization of VAC14 in HeLa cells. Fixed cells were stained with 8 μg/mL Anti-VAC14 (<B>Cat. No. SAB4200074</B>) followed by Goat Anti-Rabbit IgG, Cy3 conjugate (×40 magnification). Nuclear staining with DAPI.

Immunofluorescence
Cytoplasmic and nuclear localization of VAC14 in HeLa cells. Fixed cells were stained with 8 μg/mL Anti-VAC14 (Cat. No. SAB4200074) followed by Goat Anti-Rabbit IgG, Cy3 conjugate (×40 magnification). Nuclear staining with DAPI.

<B>Immunoprecipitation</B><BR/>Anti-VAC14 (<B>Cat. No. SAB4200074</B>), was used to immunoprecipitate human VAC14 from A431 cell lysate. Detection antibody: Anti-VAC14 (C-terminal) (<B>Cat. No. SAB4200075</B>).<BR/><B>Lanes</B><BR/>1. IP antibody: 10 μg, (50 μg cell lysate)<BR/>2. IP antibody: 10 μg, (30 μg cell lysate)<BR/>3. Negative control: without IP antibody<BR/>4. Negative control: without cell lysate<BR/>5. IB control: A431 cell lysate. Detection antibody: Anti-VAC14 (<B>Cat. No. SAB4200074</B>)

Immunoprecipitation
Anti-VAC14 (Cat. No. SAB4200074), was used to immunoprecipitate human VAC14 from A431 cell lysate. Detection antibody: Anti-VAC14 (C-terminal) (Cat. No. SAB4200075).
Lanes
1. IP antibody: 10 μg, (50 μg cell lysate)
2. IP antibody: 10 μg, (30 μg cell lysate)
3. Negative control: without IP antibody
4. Negative control: without cell lysate
5. IB control: A431 cell lysate. Detection antibody: Anti-VAC14 (Cat. No. SAB4200074)

<B>Immunoblotting</B><BR/>Cell extracts were separated on SDS-PAGE, blotted with 1.5 μg/mL Anti-VAC14 (<B>Cat. No. SAB4200074</B>), and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. HeLa<BR/>2. HEK293-T<BR/>3. HepG2<BR/>4. A431<BR/>5. HeLa. Antibody + VAC14 immunizing peptide (human, 341-359)

Immunoblotting
Cell extracts were separated on SDS-PAGE, blotted with 1.5 μg/mL Anti-VAC14 (Cat. No. SAB4200074), and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. HeLa
2. HEK293-T
3. HepG2
4. A431
5. HeLa. Antibody + VAC14 immunizing peptide (human, 341-359)