Anti-VPS4 antibody produced in rabbit

Name	Anti-VPS4 antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	SAB4200025
Prices	$61.10, $360.00
Sizes	25 µl, 200 µl
Host	Rabbit
Clonality	Polyclonal
Applications	IP WB
Species Reactivities	Human, Mouse, Rat
Antigen	synthetic peptide corresponding to amino acids 422-437 of human VPS4A, conjugated to KLH
Description	Rabbit Polyclonal
Gene	VPS4A
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunoprecipitation</B><BR/>Rabbit Anti-VPS4 (<B>Cat. No. SAB4200025</B>) was used to immunoprecipitate VPS4 from HeLa whole cell extract. Detection antibody: Rabbit Anti-VPS4 (<B>Cat. No. SAB4200025</B>) followed by Monoclonal Anti-Rabbit IgG native-peroxidase (<B>Cat. No. R3155</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. Positive control: HeLa whole cell extract<BR/>2. 10 μg antibody <BR/>3. 2 μg antibody

Immunoprecipitation
Rabbit Anti-VPS4 (Cat. No. SAB4200025) was used to immunoprecipitate VPS4 from HeLa whole cell extract. Detection antibody: Rabbit Anti-VPS4 (Cat. No. SAB4200025) followed by Monoclonal Anti-Rabbit IgG native-peroxidase (Cat. No. R3155) and a chemiluminescent substrate.
Lanes
1. Positive control: HeLa whole cell extract
2. 10 μg antibody
3. 2 μg antibody

Immunoprecipitation
Rabbit Anti-VPS4 (Cat. No. SAB4200025) was used to immunoprecipitate VPS4 from HeLa whole cell extract. Detection antibody: Rabbit Anti-VPS4B (Cat. No. SAB4200023) followed by Monoclonal Anti-Rabbit IgG native-peroxidase (Cat. No. R3155) and a chemiluminescent substrate.
Lanes
1. 10 μg antibody
2. 2 μg antibody
3. Negative control: 10 μg nonrelevant antibody

Immunoprecipitation
Rabbit Anti-VPS4 (Cat. No. SAB4200025) was used to immunoprecipitate VPS4 from HeLa whole cell extract. Detection antibody: Rabbit Anti-VPS4A (Cat. No. SAB4200022) followed by Monoclonal Anti-Rabbit IgG native-peroxidase (Cat. No. R3155) and a chemiluminescent substrate.
Lanes
1. 10 μg antibody
2. 2 μg antibody
3. Negative control: 10 μg nonrelevant antibody

<B>Immunoblotting</B><BR/>Whole extract of NRK cells was separated on SDS-PAGE and probed with Rabbit Anti-VPS4 (<B>Cat. No. SAB4200025</B>). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. 2 μg/mL antibody<BR/>2. 2 μg/mL antibody + VPS4A immunizing peptide (human, 422-437) <BR/>3. 1 μg/mL antibody

Immunoblotting
Whole extract of NRK cells was separated on SDS-PAGE and probed with Rabbit Anti-VPS4 (Cat. No. SAB4200025). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. 2 μg/mL antibody
2. 2 μg/mL antibody + VPS4A immunizing peptide (human, 422-437)
3. 1 μg/mL antibody

<B>Immunoblotting</B><BR/>Whole extract of 3T3 cells was separated on SDS-PAGE and probed with Rabbit Anti-VPS4 (<B>Cat. No. SAB4200025</B>). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. 2 μg/mL antibody<BR/>2. 2 μg/mL antibody + VPS4A immunizing peptide (human, 422-437) <BR/>3. 1 μg/mL antibody

Immunoblotting
Whole extract of 3T3 cells was separated on SDS-PAGE and probed with Rabbit Anti-VPS4 (Cat. No. SAB4200025). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. 2 μg/mL antibody
2. 2 μg/mL antibody + VPS4A immunizing peptide (human, 422-437)
3. 1 μg/mL antibody

<B>Immunoblotting</B><BR/>Whole extract of HeLa cells was separated on SDS-PAGE and probed with Rabbit Anti-VPS4 (<B>Cat. No. SAB4200025</B>). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. 2 μg/mL antibody<BR/>2. 2 μg/mL antibody + VPS4A immunizing peptide (human, 422-437) <BR/>3. 1 μg/mL antibody

Immunoblotting
Whole extract of HeLa cells was separated on SDS-PAGE and probed with Rabbit Anti-VPS4 (Cat. No. SAB4200025). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. 2 μg/mL antibody
2. 2 μg/mL antibody + VPS4A immunizing peptide (human, 422-437)
3. 1 μg/mL antibody