ImmunofluorescenceAnti-ACADSB (316-330):
Cat. No. A5109: Immunofluorescence of HUVEC cells using ACADSB (316-330), Cat. No. A5109 (red) at a 1:100 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com
ImmunoblottingLysate of HEK-293T cells overexpressing human ACADSB was separated on SDS-PAGE, blotted with Anti-ACADSB (316-330) (
Cat. No. A5109) and developed using Goat Anti-Rabbit IgG-Peroxidase (
Cat. No. A0545) and a chemiluminescent substrate.
Lanes1. Antibody dilution 1:1,000
2. Antibody dilution 1:1,000 + ACADSB immunizing peptide (human, 316-330)
ChartAnti-ACADSB (316-330):
Cat. No. A5109: Intensity analysis of Anti-ACADSB (316-330) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.