Anti-DLC1 (C-terminal region) antibody produced in rabbit

Name	Anti-DLC1 (C-terminal region) antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	SAB4200490
Prices	$337.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal
Applications	IHC IP ICC/IF WB
Species Reactivities	Human
Antigen	synthetic peptide corresponding to a sequence near the C-terminal of human DLC1 isoform 1, conjugated to KLH
Description	Rabbit Polyclonal
Gene	DLC1
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunohistochemistry</B><BR/>Localization of DLC1 in human colon. Formalin-fixed paraffin-embedded tissue was stained with 10 μg/mL. Anti-DLC1 (C-terminal region) (<B>Cat. No. SAB4200490</B>) followed by Biotin-Goat Anti-Rabbit IgG/ExtrAvidin<SUP>®</SUP>HRP (×20 magnification).

Immunohistochemistry
Localization of DLC1 in human colon. Formalin-fixed paraffin-embedded tissue was stained with 10 μg/mL. Anti-DLC1 (C-terminal region) (Cat. No. SAB4200490) followed by Biotin-Goat Anti-Rabbit IgG/ExtrAvidin^®HRP (×20 magnification).

<B>Immunofluorescence</B><BR/>Cytoplasmic and membrane localization of DLC1 in MDBK cells. Fixed cells were stained with 1 μg/mL Anti-DLC1 (C-terminal region) (<B>Cat. No. SAB4200490</B>) followed by Goat Anti-Rabbit IgG, Cy3 conjugate (×40 magnification). Nuclear staining with DAPI.

Immunofluorescence
Cytoplasmic and membrane localization of DLC1 in MDBK cells. Fixed cells were stained with 1 μg/mL Anti-DLC1 (C-terminal region) (Cat. No. SAB4200490) followed by Goat Anti-Rabbit IgG, Cy3 conjugate (×40 magnification). Nuclear staining with DAPI.

<B>Immunoblotting</B><BR/>Cell lysates were separated on SDS-PAGE, blotted with 1.5 μg/mL Anti-DLC1 (C-terminal region) (<B>Cat. No. SAB4200490</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. HEK293-T overexpressing human DLC1<BR/>2. HEK293-T non-transfected<BR/>3. HEK293-T overexpressing human DLC1 + immunizing DLC1 peptide

Immunoblotting
Cell lysates were separated on SDS-PAGE, blotted with 1.5 μg/mL Anti-DLC1 (C-terminal region) (Cat. No. SAB4200490) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. HEK293-T overexpressing human DLC1
2. HEK293-T non-transfected
3. HEK293-T overexpressing human DLC1 + immunizing DLC1 peptide

<B>Immunoprecipitation</B><BR/>Anti-DLC1 (C-terminal region) (<B>Cat. No. SAB4200490</B>), was used to immunoprecipitate DLC1 from HEK293-T cells overexpressing human DLC1. Detection antibody: Anti-DLC1 (<B>Cat. No. SAB4200351</B>).<BR/><B>Lanes</B><BR/>1. IP antibody: 5 μg<BR/>2. Negative control: without cell lysate<BR/>3. Negative control: without IP antibody

Immunoprecipitation
Anti-DLC1 (C-terminal region) (Cat. No. SAB4200490), was used to immunoprecipitate DLC1 from HEK293-T cells overexpressing human DLC1. Detection antibody: Anti-DLC1 (Cat. No. SAB4200351).
Lanes
1. IP antibody: 5 μg
2. Negative control: without cell lysate
3. Negative control: without IP antibody