Anti-WTX (N-terminal) antibody produced in rabbit

Name	Anti-WTX (N-terminal) antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	SAB4200184
Prices	$322.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal
Applications	WB
Species Reactivities	Human
Antigen	synthetic peptide corresponding to amino acids 20-39 of human WTX (GeneID: 139285), conjugated to KLH
Description	Rabbit Polyclonal
Gene	AMER1
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunoblotting</B><BR/>Cell lysates of HEK-293T overexpressing human WTX (lanes 1,2) and HEK-293T non-transfected (lane 3) were separated on SDS-PAGE, blotted with Anti-WTX (N-terminal) (<B>Cat. No. SAB4200184</B>), and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. Bands at 190 kDa and 150 kDa respectively correspond to the unspliced and spliced isoforms of WTX.<BR/><B>Lanes</B><BR/>1. Antibody: 1 μg/mL <BR/>2. Antibody: 1 μg/mL + immunizing WTX peptide<BR/>3. Antibody: 1 μg/mL

Immunoblotting
Cell lysates of HEK-293T overexpressing human WTX (lanes 1,2) and HEK-293T non-transfected (lane 3) were separated on SDS-PAGE, blotted with Anti-WTX (N-terminal) (Cat. No. SAB4200184), and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate. Bands at 190 kDa and 150 kDa respectively correspond to the unspliced and spliced isoforms of WTX.
Lanes
1. Antibody: 1 μg/mL
2. Antibody: 1 μg/mL + immunizing WTX peptide
3. Antibody: 1 μg/mL