AMER1

Gene Symbol	AMER1
Entrez Gene	139285
Alt Symbol	FAM123B, OSCS, WTX
Species	Human
Gene Type	protein-coding
Description	APC membrane recruitment protein 1
Other Description	RP11-403E24.2\|Wilms tumor gene on the X chromosome protein\|Wilms tumor on the X\|adenomatous polyposis coli membrane recruitment 1\|family with sequence similarity 123B\|protein FAM123B
Swissprots	Q5JTC6 Q8N885 A2IB86
Accessions	EAX05415 Q5JTC6 AK097146 BAC04964 AL833759 DA939151 EF186024 ABM60755 XM_011530858 XP_011529160 NM_152424 NP_689637
Function	Regulator of the canonical Wnt signaling pathway. Acts by specifically binding phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2), translocating to the cell membrane and interacting with key regulators of the canonical Wnt signaling pathway, such as components of the beta-catenin destruction complex. Acts both as a positive and negative regulator of the Wnt signaling pathway, depending on the context: acts as a positive regulator by promoting LRP6 phosphorylation. Also acts as a negative regulator by acting as a scaffold protein for the beta- catenin destruction complex and promoting stabilization of Axin at the cell membrane. Promotes CTNNB1 ubiquitination and degradation. Involved in kidney development. {ECO:0000269\|PubMed:17510365, ECO:0000269\|PubMed:17925383, ECO:0000269\|PubMed:19416806, ECO:0000269\|PubMed:21304492, ECO:0000269\|PubMed:21498506}.
Subcellular Location	Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Nucleus. Note=Shuttles between nucleus and cytoplasm. Detected in nuclear paraspeckles that are found close to splicing speckles. Translocates to the cell membrane following binding to PtdIns(4,5)P2.
Tissue Specificity	Detected in fetal and adult kidney, brain and spleen. {ECO:0000269\|PubMed:19416806}.

WTX (D38E5) Rabbit mAb - 5854 from Cell Signaling Technology

Prices	$246.00
Sizes	100 µl (10 western blots)
Host	Rabbit
Clonality	Monoclonal
Clone	D38E5

Western blot analysis of extracts from HeLa cells, untransfected or transfected with a contruct overexpressing human WTX, using WTX (D38E5) Rabbit mAb.

WTX (E-14) - sc-138078 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

WTX (H-120) - sc-368736 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Rabbit
Clonality	Polyclonal

WTX (K-14) - sc-138079 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

Anti-FAM123B antibody - ab198901 from Abcam

Prices	$376.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Immunohistochemical analysis of paraffin-embedded Human gastric cancer tissue labeling FAM123B with ab198901 at 1/40 dilution.

Anti-FAM123B antibody - ab91309 from Abcam

Prices	$313.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

Anti-FAM123B antibody (ab91309) at 1/50 dilution + Mouse kidney tissue lysate at 35 µg

Anti-FAM123B / WTX Antibody (aa681-708) - LS-C99281 from LifeSpan Bioscience

Prices	$295.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

WTX Antibody IHC of formalin-fixed and paraffin-embedded mouse brain tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Anti-FAM123B / WTX Antibody (N-Terminus, clone D38E5) - LS-C184163 from LifeSpan Bioscience

Prices	$435.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	D38E5

WTX Antibody (729419) - MAB7374 from Novus Biologicals

Prices	$99.00, $249.00
Sizes	25 µg, 100 µg
Host	Mouse
Clonality	Monoclonal
Clone	729419

Western blot shows lysates of U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human WTX Monoclonal Antibody (Catalog # MAB7374) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for WTX at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

FAM123B Polyclonal Antibody - bs-6126R from Bioss

Prices	$269.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-WTX (C-terminal) antibody produced in rabbit - SAB4200163 from Sigma-Aldrich

Prices	$55.60, $322.00
Sizes	25 µl, 200 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Cell lysates of HEK-293T overexpressing human WTX (lanes 1, 2) and HEK-293T non-transfected (lane 3) were separated on SDS-PAGE, blotted with Anti-WTX (C-terminal) (<B>Cat. No. SAB4200163</B>), and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. Bands at 190 kDa and 150 kDa respectively correspond to the unspliced and spliced isoforms of WTX.<BR/><B>Lanes</B><BR/>1. Antibody: 1 μg/mL <BR/>2. Antibody: 1 μg/mL + WTX immunizing peptide (human, 1119-1135) <BR/>3. Antibody: 1 μg/mL

Anti-WTX antibody produced in rabbit - SAB4200162 from Sigma-Aldrich

Prices	$55.60, $322.00
Sizes	25 µl, 200 µl
Host	Rabbit

ANTI-WTX (CENTER) antibody produced in rabbit - SAB1306508 from Sigma-Aldrich

Prices	$326.00
Sizes	40 tst
Host	Rabbit
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Anti-WTX (Center): <B>Cat. No. SAB1306508</B>: Western blot analysis of WTX Antibody (Center) in Mouse kidney tissue lysates (35 μg/lane).

Anti-WTX (N-terminal) antibody produced in rabbit - SAB4200184 from Sigma-Aldrich

Prices	$322.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-AMER1 antibody produced in rabbit - SAB4301079 from Sigma-Aldrich

Prices	$230.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

FAM123B Antibody - middle region (OAAB03558) - OAAB03558 from Aviva Systems Biology

Prices	$289.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

WTX-Antibody-Center - AP6553c from Abgent, a WuXi AppTec company

Prices	$99.00, $295.00
Sizes	80 µl, 400 µl
Host	Rabbit
Clonality	Polyclonal

Western blot analysis of WTX antibody (Center) (Cat. #AP6553c) in mouse kidney tissue lysates (35ug/lane). WTX (arrow) was detected using the purified Pab.

Human WTX Antibody - MAB7374 from R&D Systems

Prices	$99.00, $249.00
Sizes	25 µg, 100 µg
Host	Mouse
Clone	729419

Detection of Human WTX by Western Blot. Western blot shows lysates of U937 human histiocytic lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human WTX Monoclonal Antibody (Catalog # MAB7374) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007 ). A specific band was detected for WTX at approximately 190 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1 .

Human WTX MAb (Clone 729419), Mouse IgG2B - MAB7374-SP from R&D Systems

Host	Mouse
Clonality	Monoclonal
Clone	729419

FAM123B antibody (FITC) - orb102568 from Biorbyt

Prices	$315.00
Sizes	100 μg
Host	Rabbit
Clonality	Polyclonal

WTX Antibody - abx032867 from Abbexa

Host	Rabbit
Clonality	Polyclonal

WTX antibody - 55432-1-AP from Acris Antibodies

Prices	$270.00
Sizes	150 µl
Host	Rabbit
Clonality	Polyclonal

Immunohistochemistry of paraffin-embedded human kidney tissue slide using 55432-1-AP( FAM123B Antibody) at dilution of 1:200 (under 10x lens)

AMER1 (Center) antibody - AP17838PU-N from Acris Antibodies

Prices	$330.00
Sizes	400 µl
Host	Rabbit
Clonality	Polyclonal

Western blot analysis of WTX antibody (Center) (AP17838PU-N) in mouse kidney tissue lysates (35ug/lane). WTX (arrow) was detected using the purified Pab.

Rabbit Anti-WTX (Center) - 102-16820 from RayBiotech

Host	Rabbit

Western blot analysis of WTX antibody (Center) (Cat# 102-16820) in mouse kidney tissue lysates (35ug/lane). WTX (arrow) was detected using the purified Pab.

WTX antibody (Center) - STJ50026 from St John's Laboratory Ltd

Host	Rabbit
Clonality	Polyclonal
Clone	RB18795

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