ImmunofluorescencePSPC1 staining in HEK-293T cells. Cells were fixed and stained with1.2 μg/mL Rabbit Anti-PSPC1 (N-Terminal) (
Cat. No. SAB4200068) followed by Goat Anti-Rabbit, Cy3 conjugate (×100 magnification).
ImmunoblottingLysate of HEK-293T cells was separated on SDS-PAGE and probed with Rabbit Anti-PSPC1 (N-Terminal) (
Cat. No. SAB4200068). The antibody was developed with Goat Anti-Rabbit IgG, peroxidase conjugate (
Cat. No. A0545) and a chemiluminescence substrate.
Lanes1. Antibody 2 μg/mL
2. Antibody 1 μg/ mL
3. Antibody 0.5 μg/ mL
4. Antibody 1 μg/mL + 10 μg/mL PSPC1 immunizing peptide
ImmunoblottingLysates of the indicated cells were separated on SDS-PAGE and probed with 2 μg/mL Rabbit Anti-PSPC1 (N-Terminal) (
Cat. No. SAB4200068). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (
Cat. No. A0545) and a chemiluminescence substrate.
Lanes1. HeLa; 2. K-562; 3. Raw264; 4. HEK-293T; 5. HEK-293T over-expressing PSPC1