PSPC1

Gene Symbol PSPC1
Entrez Gene 55269
Alt Symbol PSP1
Species Human
Gene Type protein-coding
Description paraspeckle component 1
Other Description paraspeckle protein 1
Swissprots Q8WXE8 Q9NV36 Q8WXF1 Q5JTQ3 Q8NCZ9
Accessions EAX08232 EAX08233 EAX08234 Q8WXF1 AF448795 AAL59601 AF449627 AAL59602 AK001817 BAA91924 AK090873 AK301554 BAG63050 AL834505 CAD39162 BC014184 AAH14184 BM981042 BP225439 BQ685330 CA425205 CB214993 CR457272 CAG33553 CX759783 DA644474 DC396174 DQ892679 ABM83605 DQ895922 ABM86848 XM_006719844 XP_006719907 XM_011535137 XP_011533439 XM_011535138 XP_011533440 XM_011535139 XP_011533441 XM_011535140 XP_011533442 XM_011535141 XP_011533443 XM_011535142 XP_011533444 XM_011535143 XP_011533445 XR_941616 XR_941617 XR_941619 NM_001042414 NP_001035879 NR_003272 NR_044998
Function Regulates, cooperatively with NONO and SFPQ, androgen receptor-mediated gene transcription activity in Sertoli cell line (By similarity). Binds to poly(A), poly(G) and poly(U) RNA homopolymers. Regulates the circadian clock by repressing the transcriptional activator activity of the CLOCK-ARNTL/BMAL1 heterodimer (By similarity). Together with NONO, required for the formation of nuclear paraspeckles. {ECO:0000250, ECO:0000269|PubMed:22416126}.
Subcellular Location Nucleus, nucleolus. Nucleus matrix {ECO:0000250}. Cytoplasm {ECO:0000250}. Nucleus speckle. Note=In punctate subnuclear structures often located adjacent to splicing speckles, called paraspeckles. Colocalizes with NONO and SFPQ in paraspeckles and perinucleolar caps in an RNA-dependent manner. May cycles between paraspeckles and nucleolus. In telophase, when daughter nuclei form, localizes to perinucleolar caps.
Tissue Specificity Expressed in pancreas, kidney, skeletal muscle, liver, lung, placenta, brain and heart. {ECO:0000269|PubMed:11790299}.

PSPC1 (G-7) - sc-374387 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PSPC1 (G-7): sc-374387. Western blot analysis of PSPC1 expression in T24 whole cell lysate.

PSPC1 (T-20) - sc-84577 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
PSPC1 (T-20): sc-84577. Western blot analysis of PSPC1 expression in HeLa whole cell lysate (A) and mouse thymus tissue extract (B).

PSPC1 (C-3) - sc-374181 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Mouse
Clonality Monoclonal
PSPC1 (C-3): sc-374181. Western blot analysis of PSPC1 expression in HeLa whole cell lysate.

PSPC1 (N-16) - sc-84576 from Santa Cruz Biotechnology

Prices $279.00
Sizes 100 µg/ml
Host Rabbit
Clonality Polyclonal
PSPC1 (N-16): sc-84576. Western blot analysis of PSPC1 expression in K-562 (A), Ramos (B), BJAB (C) and Jurkat (D) nuclear extracts.

Anti-PSPC1 antibody - ab104238 from Abcam

Prices $384.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
PSPC1 was immunoprecipitated using 0.5mg Hek293 whole cell extract, 5µg of Rabbit polyclonal to and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, Hek293 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab104238.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 62kDa, non specific bands - 50 and 55kDa: We are unsure as to the identity of this extra band; PSPC1

Anti-PSPC1 antibody - ab184123 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PSPC1 antibody (ab184123) at 1/2000 dilutionLane 1 : 293T whole cell lysateLane 2 : Hela whole cell lysateLane 3 : HepG2 whole cell lysateLysates/proteins at 30 µg per lane.

Anti-PSPC1 antibody - ab176694 from Abcam

Prices $376.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Detection of Human PSPC1 by Western Blot of Immunoprecipitates. 1 mg for IP; 20% of IP HeLa whole cell lysate loaded. ab176694 used for IP at 6 µg/mg lysate. For blotting immunoprecipitated PSPC1, ab176694 was used at 0.4 µg/ml.

Anti-PSPC1 antibody - ab115184 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-PSPC1 antibody (ab115184) at 0.04 µg/mlLane 1 : HeLa whole cell lysate at 50 µgLane 2 : HeLa whole cell lysate at 15 µgLane 3 : 293T whole cell lysate at 50 µgLane 4 : Jurkat whole cell lysate at 50 µgdeveloped using the ECL technique

Anti-PSPC1 Antibody (aa1-50) - LS-C289900 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PSPC1 Antibody (aa425-475) - LS-C289901 from LifeSpan Bioscience

Prices $375.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

PSPC1 Antibody - NBP1-83801 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-PSPC1 (N-terminal) antibody produced in rabbit - SAB4200068 from Sigma-Aldrich

Prices $61.10, $360.00
Sizes 25 µl, 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunofluorescence</B><BR/>PSPC1 staining in HEK-293T cells. Cells were fixed and stained with1.2 μg/mL Rabbit Anti-PSPC1 (N-Terminal) (<B>Cat. No. SAB4200068</B>) followed by Goat Anti-Rabbit, Cy3 conjugate (×100 magnification).

Anti-PSPC1 (C-terminal) antibody produced in rabbit - SAB4200067 from Sigma-Aldrich

Prices $354.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunofluorescence</B><BR/>Staining of PSPC1 in HEK-293T cells. Cells were fixed and stained with 1.25 μg/mL Rabbit Anti-PSPC1 (C-Terminal) (<B>Cat. No. SAB4200067</B>) followed by Goat Anti-Rabbit, Cy3 conjugate and counterstained with DAPI (blue) to stain nuclei (×40 magnification).

Anti-PSPC1 antibody produced in rabbit - HPA038904 from Sigma-Aldrich

Prices $429.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Anti-PSPC1: <B>Cat. No. HPA038904</B>: Immunohistochemical staining of human placenta shows strong nuclear positivity in trophoblastic cells.

PSPC1 / PSP1 antibody - 16714-1-AP from Acris Antibodies

Prices $270.00
Sizes 150 µl
Host Rabbit
Clonality Polyclonal
PC-3 cells were subjected to SDS PAGE followed by western blot with 16714-1-AP( PSPC1 Antibody) at dilution of 1:1000

PSPC1 antibody - 16714-1-AP from Proteintech Group

Prices $279.00
Sizes con: 119 μg/150μl
Host Rabbit
Clonality Polyclonal
PC-3 cells were subjected to SDS PAGE followed by western blot with 16714-1-AP( PSPC1 Antibody) at dilution of 1:1000

Anti-PSPC1 (aa 1-50) polyclonal antibody - DPABH-12051 from Creative Diagnostics

Host Rabbit
Clonality Polyclonal

Anti-PSPC1 (aa 1-176) polyclonal antibody - DPABH-14578 from Creative Diagnostics

Host Rabbit
Clonality Polyclonal

Anti-PSPC1 (aa 50-150) polyclonal antibody - DPABH-18292 from Creative Diagnostics

Host Rabbit
Clonality Polyclonal

PSP1 Antibody - A303-205A from Bethyl Labs

Prices $99.00, $229.00, $339.00
Sizes 20 µl (2 blots), 100 µl (10 blots), 100 µl (0.2 mg/ml)
Host Rabbit
Clonality Polyclonal

PSP1 Antibody - A303-206A from Bethyl Labs

Prices $99.00, $229.00, $339.00
Sizes 20 µl (2 blots), 100 µl (10 blots), 100 µl (1 mg/ml)
Host Rabbit
Clonality Polyclonal

PSP1 Antibody AbVantage™ Pack - A311-182A from Bethyl Labs

Prices $472.00
Sizes 1 pack
Host Rabbit
Clonality Polyclonal

PSPC1 Polyclonal Antibody - PA5-35958 from Thermo Fisher Scientific

Prices $309.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Immunohistochemical analysis of PSPC1 in the nucleus of paraffin-embedded rat fore brain tissue using a PSPC1 polyclonal antibody (Product # PA5-35958) at a dilution of 1:500.

PSPC1 antibody - GTX119744 from GeneTex

Prices $119.00
Sizes 25 μl
Host Rabbit
Clonality Polyclonal
Anti-PSPC1 antibody used in Western blot. GTX119744

PSPC1 Antibody - DF2220 from Affinity Biosciences

Prices $220.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
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