Anti-Derlin-3 antibody produced in rabbit

Name	Anti-Derlin-3 antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	D2194
Prices	$59.70, $346.00
Sizes	25 µl, 200 µl
Host	Rabbit
Clonality	Polyclonal
Applications	IP ICC/IF WB
Species Reactivities	Mouse, Rat, Human
Antigen	synthetic peptide corresponding to amino acid residues 192-205 of human Derlin-3, conjugated to KLH
Description	Rabbit Polyclonal
Gene	DERL3
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunofluorescence</B><BR/>HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 20 μg/mL Rabbit Anti-Derlin-3 (<B>Cat. No. D2194</B>). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate.

Immunofluorescence
HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 20 μg/mL Rabbit Anti-Derlin-3 (Cat. No. D2194). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate.

<B>Immunoprecipitation</B><BR/>Rabbit Anti-Derlin-3 (<B>Cat. No. D2194</B>) was used to immunoprecipitate Derlin-3 from HEK-293T overexpressing human Derlin-3 whole cell extract. Detection antibody: Mouse Anti-c-Myc-antibody (<B>Cat. No. M4439</B>)<BR/><B>Lanes</B><BR/>1. 2 μg antibody <BR/>2. 5 μg antibody<BR/>3. 5 μg non-relevant antibody

Immunoprecipitation
Rabbit Anti-Derlin-3 (Cat. No. D2194) was used to immunoprecipitate Derlin-3 from HEK-293T overexpressing human Derlin-3 whole cell extract. Detection antibody: Mouse Anti-c-Myc-antibody (Cat. No. M4439)
Lanes
1. 2 μg antibody
2. 5 μg antibody
3. 5 μg non-relevant antibody

<B>Immunoblotting</B><BR/>Whole extract of HEK-293T cells overexpressing human Derlin-3 was separated on SDS-PAGE and probed with Rabbit Anti-Derlin-3 (<B>Cat. No. D2194</B>). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate.<BR/><B>Lanes</B><BR/>1. 5 μg/mL antibody <BR/>2. 5 μg/mL antibody + Derlin-3 immunizing peptide (human, 192-205)

Immunoblotting
Whole extract of HEK-293T cells overexpressing human Derlin-3 was separated on SDS-PAGE and probed with Rabbit Anti-Derlin-3 (Cat. No. D2194). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. 5 μg/mL antibody
2. 5 μg/mL antibody + Derlin-3 immunizing peptide (human, 192-205)

Product References

Multiprotein complexes that link dislocation, ubiquitination, and extraction of - Multiprotein complexes that link dislocation, ubiquitination, and extraction of

Lilley BN, Ploegh HL. Proc Natl Acad Sci U S A. 2005 Oct 4;102(40):14296-301. Epub 2005 Sep 26.

Derlin-2 and Derlin-3 are regulated by the mammalian unfolded protein response - Derlin-2 and Derlin-3 are regulated by the mammalian unfolded protein response

Oda Y, Okada T, Yoshida H, Kaufman RJ, Nagata K, Mori K. J Cell Biol. 2006 Jan 30;172(3):383-93.