ImmunoblottingCell line lysates were separated on SDS-PAGE and probed with 5 μg/mL Rabbit Anti-Strumpellin (C-terminal) (
Cat. No. SAB4200550). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (
Cat. No. A0545) and a chemiluminescent substrate.
Lanes1. PC-3
2. HeLa
3. HepG2
ImmunoprecipitationRabbit Anti-Strumpellin (C-terminal) (
Cat. No. SAB4200550) was used to immunoprecipitate Strumpellin from a whole extract of DU-145 cells. Detection antibody: Rabbit Anti-strumpellin (
Cat. No. SAB4200549).
Lanes1. 5 μg antibody
2. 10 μg antibody
3. Negative control: without 1st antibody
4. Positive control: Du-145 whole cell extract