ImmunofluorescenceHeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton
™ X-100. Fixed cells were stained with 1 μg/mL Rabbit Anti-PLAUR (
Cat. No. SAB4200358). The antibody was developed using Goat Anti-Rabbit IgG, Atto488 conjugate (
Cat. No. 18772).
ImmunoblottingWhole extract of HEK-293T cells overexpressing human PLAUR cells was separated on SDS-PAGE and probed with Rabbit Anti-PLAUR (
Cat. No. SAB4200358). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (
Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. 0.5 μg/mL antibody
2. 1 μg/mL antibody
3. 1 μg/mL antibody + PLAUR immunizing peptide