ImmunofluorescenceCytoplasmic and perinuclear localization of ALS2CL in A431 cells. Fixed cells were stained with 1 μg/mL Anti-ALS2CL (N-terminal) (
Cat. No. SAB4200165) followed by Goat Anti-Rabbit IgG, Cy3 conjugate (×40 magnification). Nuclear staining with DAPI.
ImmunoblottingCell lysates were separated on SDS-PAGE, blotted with 1.5 μg/mL Anti-ALS2CL (N-terminal) (
Cat. No. SAB4200165), and developed using Goat Anti-Rabbit IgG-Peroxidase (
Cat. No. A0545) and a chemiluminescent substrate.
Lanes1. SH-SY5Y; 2. A431; 3. HepG2; 4. HepG2 + ALS2CL immunizing peptide (mouse, 117-134)