Anti-CLK4 (61-75) antibody produced in rabbit

Name	Anti-CLK4 (61-75) antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	C2744
Prices	$342.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal
Applications	WB
Species Reactivities	Human
Antigen	synthetic peptide corresponding to amino acids 61-75 of human CLK4
Description	Rabbit Polyclonal
Gene	CLK4
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunofluorescence</B><BR/>Anti-CLK4 (61-75) (RB): <B>Cat. No. C2744</B>: Immunofluorescence of HUVEC cells using CLK4 (61-75) (RB), <B>Cat. No. C2744</B> (red) at a 1:100 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue). Yale HTCB IF procedure used.<BR/>Images have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Immunofluorescence
Anti-CLK4 (61-75) (RB): Cat. No. C2744: Immunofluorescence of HUVEC cells using CLK4 (61-75) (RB), Cat. No. C2744 (red) at a 1:100 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue). Yale HTCB IF procedure used.
Images have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human CLK4 was separated on SDS-PAGE, blotted with Anti-CLK4 (61-75) (<B>Cat. No. C2744</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. Antibody dilution 1:500

Immunoblotting
Lysate of HEK-293T cells overexpressing human CLK4 was separated on SDS-PAGE, blotted with Anti-CLK4 (61-75) (Cat. No. C2744) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. Antibody dilution 1:500

<B>Chart</B><BR/>Anti-CLK4 (61-75) (RB): <B>Cat. No. C2744</B>: Intensity analysis of Anti-CLK4 (61-75) (RB) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.

Chart
Anti-CLK4 (61-75) (RB): Cat. No. C2744: Intensity analysis of Anti-CLK4 (61-75) (RB) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.

Product References

Molecular characterization of a cDNA encoding functional human CLK4 kinase and - Molecular characterization of a cDNA encoding functional human CLK4 kinase and

Schultz J, Jones T, Bork P, Sheer D, Blencke S, Steyrer S, Wellbrock U, Bevec D, Ullrich A, Wallasch C. Genomics. 2001 Feb 1;71(3):368-70.

Structural analysis of UBL5, a novel ubiquitin-like modifier. - Structural analysis of UBL5, a novel ubiquitin-like modifier.

McNally T, Huang Q, Janis RS, Liu Z, Olejniczak ET, Reilly RM. Protein Sci. 2003 Jul;12(7):1562-6.

Novel SR-rich-related protein clasp specifically interacts with inactivated Clk4 - Novel SR-rich-related protein clasp specifically interacts with inactivated Clk4

Katsu R, Onogi H, Wada K, Kawaguchi Y, Hagiwara M. J Biol Chem. 2002 Nov 15;277(46):44220-8. Epub 2002 Aug 6.