Anti-Myocardin antibody produced in rabbit
| Name | Anti-Myocardin antibody produced in rabbit |
|---|---|
| Supplier | Sigma-Aldrich |
| Catalog | SAB4200539 |
| Prices | $337.00 |
| Sizes | 200 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Applications | IHC ICC/IF WB |
| Species Reactivities | Human, Mouse, Rat |
| Antigen | a synthetic peptide corresponding to an internal sequence of human myocardin, conjugated to KLH |
| Description | Rabbit Polyclonal |
| Gene | MYOCD |
| Conjugate | unconjugated |
| Supplier Page | Shop |
Product images
ImmunohistochemistryStaining of formalin-fixed, paraffin-embedded Human heart sections with 10 μg/mL Rabbit Anti-Myocardin (Cat. No. SAB4200539) using biotin/ExtrAvidin®-Peroxidase.
ImmunohistochemistryStaining of formalin-fixed, paraffin-embedded Chicken heart sections with 10 μg/mL Rabbit Anti-Myocardin (Cat. No. SAB4200539) using biotin/ExtrAvidin®-Peroxidase.
ImmunofluorescenceA10 cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 2 μg/mL Rabbit Anti-Myocardin (Cat. No. SAB4200539). The antibody was developed using Goat Anti-Rabbit IgG, FITC conjugate. Cells were counterstained with phalloidin rhodamine conjugate to stain Actin (red).
ImmunoblottingCell line lysates were separated on SDS-PAGE and probed with 1 μg/mL Rabbit Anti-Myocardin (Cat. No. SAB4200539). The antibody was developed using Goat Anti-Rabbit IgG-peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. HCT-116
2. HEK-293 – overexpressing Myocardin
3. SW-480
4. HCT-116 + Myocardin immunizing peptide