MYOCD

Gene Symbol MYOCD
Entrez Gene 93649
Alt Symbol MYCD
Species Human
Gene Type protein-coding
Description myocardin
Other Description -
Swissprots Q8IZQ8 Q8N7Q1 Q5UBU5
Accessions EAW89970 EAW89971 Q8IZQ8 AF532596 AAN33040 AK095846 AK096753 AK097821 BAC05177 AK128608 AK292885 BAF85574 AY764180 AAV33439 BC126307 AAI26308 BC143391 AAI43392 BQ000229 BX640673 CAE45805 DA650534 DB226467 XM_005256863 XP_005256920 XM_005256864 XP_005256921 NM_001146312 NP_001139784 NM_153604 NP_705832 NM_001146313
Function Smooth muscle cells (SM) and cardiac muscle cells- specific transcriptional factor which uses the canonical single or multiple CArG boxes DNA sequence. Acts as a cofactor of serum response factor (SRF) with the potential to modulate SRF-target genes. Plays a crucial role in cardiogenesis and differentiation of the smooth muscle cell lineage (myogenesis) (By similarity). {ECO:0000250}.
Subcellular Location Nucleus {ECO:0000250}.
Tissue Specificity Expressed in heart, aorta, and in smooth muscle cell-containing tissues: stomach, bladder, small intestine, colon, lung, placenta and uterus. Very faint expression in prostate and skeletal muscle. {ECO:0000269|PubMed:12640126, ECO:0000269|PubMed:20385216}.

Myocardin (M-16) - sc-21561 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal

Myocardin (A-13) - sc-34238 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Goat
Clonality Polyclonal
Myocardin (A-13): sc-34238. Western blot analysis of Myocardin expression in SW480 nuclear extract.

Myocardin (H-300) - sc-33766 from Santa Cruz Biotechnology

Prices $279.00
Sizes 200 µg/ml
Host Rabbit
Clonality Polyclonal
Myocardin (H-300): sc-33766. Western blot analysis of Myocardin expression in mouse heart tissue extract.

Anti-Myocardin antibody - ab107301 from Abcam

Prices $376.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
All lanes : Anti-Myocardin antibody (ab107301) at 1 µg/mlLane 1 : Small Intestine (Human) Tissue Lysate - adult normal tissue (ab29276)Lane 2 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate Lysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Myocardin antibody - ab106346 from Abcam

Prices $370.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal

Anti-Myocardin antibody - C-terminal - ab202385 from Abcam

Prices $359.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Anti-Myocardin antibody - C-terminal (ab202385) at 1/1000 dilution + mouse C2C12 cell line at 20 µgSecondaryGoat anti-rabbit IgG H&L(HRP) at 1/10000 dilution

Anti-MYOCD / Myocardin Antibody (aa97-290) - LS-C37407 from LifeSpan Bioscience

Prices $465.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal

Anti-MYOCD / Myocardin Antibody (Internal) - LS-C153495 from LifeSpan Bioscience

Prices $345.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Anti-MYOCD / Myocardin Antibody (aa237-286) - LS-C135249 from LifeSpan Bioscience

Prices $365.00
Sizes 50 µg
Host Rabbit
Clonality Polyclonal
MYOCD / Myocardin antibody LS-C135249 Western blot of Mouse Pancreas lysate. Antibody concentration 1 ug/ml.

Anti-MYOCD / Myocardin Antibody - LS-C137255 from LifeSpan Bioscience

Prices $255.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Anti-MYOCD / Myocardin Antibody (aa743-777) - LS-C344550 from LifeSpan Bioscience

Prices $295.00
Sizes 400 µl
Host Rabbit
Clonality Polyclonal
Western blot of lysate from mouse C2C12 cell line with Myocd Antibody. Antibody was diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20 ug.

Myocardin Antibody (355521) - MAB4028 from Novus Biologicals

Prices $99.00, $249.00
Sizes 25 µg, 100 µg
Host Mouse
Clonality Monoclonal
Clone 355521

Myocardin Polyclonal Antibody - bs-9472R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Formalin-fixed and paraffin embedded human kidney labeled with Rabbit Anti Myocardin Polyclonal Antibody, Unconjugated (bs-9472R) at 1:200 followed by conjugation to the secondary antibody and DAB staining

Monoclonal Anti-Myocardin antibody produced in mouse - M8948 from Sigma-Aldrich

Prices $61.10, $360.00
Sizes 25 µl, 200 µl
Host Mouse
Clonality Monoclonal
Clone MCR54
<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells expressing Myocardin was separated on SDS-PAGE and probed with Mouse Anti-myocardin Clone: MCR54 (<B>Cat. No. M8948</B>). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (<B>Cat. No. A2304</B>) and a chemiluminescent substrate.<BR/><B>Lanes</B><BR/>1. 4 μg/mL antibody <BR/>2. 4 μg/mL antibody +10 μg/mL Myocardin (884-901) immunizing peptide<BR/>3. 4 μg/mL antibody +10 μg/mL Myocardin (39-58) immunizing peptide

Anti-Myocardin antibody produced in rabbit - SAB4200539 from Sigma-Aldrich

Prices $337.00
Sizes 200 µl
Host Rabbit
Clonality Polyclonal
<B>Immunohistochemistry</B><BR/>Staining of formalin-fixed, paraffin-embedded Human heart sections with 10 μg/mL Rabbit Anti-Myocardin (<B>Cat. No. SAB4200539</B>) using biotin/ExtrAvidin<SUP>®</SUP>-Peroxidase.

Human/Mouse Myocardin Antibody - MAB4028 from R&D Systems

Prices $99.00, $249.00
Sizes 25 µg, 100 µg
Host Mouse
Clone 355521
Detection of Human Myocardin by Western Blot. Western blot shows lysates of MCF-7 human breast cancer cell line, Raji human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Human Myocardin Monoclonal Antibody (Catalog # MAB4028) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007 ). A specific band was detected for Myocardin at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1 .

Human/Mouse Myocardin MAb (Clone 355521), Mouse IgG2B - MAB4028-SP from R&D Systems

Host Mouse
Clonality Monoclonal
Clone 355521
Detection of Human Myocardin by Western Blot. Western blot shows lysates of MCF‑7 human breast cancer cell line, Raji human Burkitt's lymphoma cell line, HeLa human cervical epithelial carcinoma cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 1 µg/mL of Human Myocardin Monoclonal Antibody (Catalog # MAB4028) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Myocardin at approximately 105 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

MYOCD Antibody (OABF01900) - OABF01900 from Aviva Systems Biology

Prices $289.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Myocardin antibody - orb77213 from Biorbyt

Prices $425.00
Sizes 100 μg
Host Rabbit
Clonality Polyclonal
Western blot analysis of rat brain lysates using Myocardin antibody

Myocardin antibody - 70R-18729 from Fitzgerald

Prices $375.00
Sizes 50 µl
Host Rabbit
Clonality Polyclonal

MHC class I Polyclonal Antibody - bs-7335R from Bioss

Prices $269.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

Myocardin Antibody - abx037933 from Abbexa

Host Rabbit
Clonality Polyclonal

Myocardin antibody - AP55091PU-N from Acris Antibodies

Prices $480.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

Myocardin, Monoclonal Antibody - MBS600863 from MyBioSource

Prices $605.00
Sizes 100 µg
Host Mouse
Clonality Monoclonal
Clone 7H124

Myocardin Antibody - 252149 from ABBIOTEC

Prices $292.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
The Myocardin Antibody is used in Western blot to detect myocardin in rat brain lysates.
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