ImmunofluorescenceNRK cells were fixed and permeabilized with cold methanol followed by cold acetone. Fixed cells were stained with 5 μg/mL Rabbit Anti-XTP3-B (
Cat. No. SAB4200062). The antibody was developed using Goat Anti-Rabbit IgG, Atto 488 conjugate (
Cat. No.18772).
ImmunoblottingCell line lysates were separated on SDS-PAGE and probed with 4 μg/mL Rabbit Anti-XTP3-B (
Cat. No. SAB4200062). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (
Cat. No. A0545) and a chemiluminescent substrate.
Lanes1. HEK-293T cells overexpressing FLAG-tagged human XTP3-B
2. HeLa
3. G361
4. PC12
5. NIH-3T3