ImmunoblottingWhole extract of HEK-293T cells overexpressing mouse WASH1 was separated on SDS-PAGE and probed with Rabbit Anti-WASH1 (
Cat. No. SAB4200372). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (
Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. 2 μg/mL antibody
2. 4 μg/mL antibody
ImmunoblottingWhole extracts of WT MEF cells (WASH +/+) or WASHout MEF cells (WASH-/-) were separated on SDS-PAGE and probed with 1:500 dilution of Rabbit Anti-WASH1 (
Cat. No. SAB4200372). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase and a chemiluminescent substrate. The picture is courtesy of Timothy S. Gomez and Daniel D. Billadeau, Departments of Biochemistry and Molecular Biology and Immunology, Division of Oncology Research, Mayo Clinic, Rochester, USA.
ImmunoprecipitationRabbit Anti-WASH1 (
Cat. No. SAB4200372) was used to immunoprecipitate WASH1 from HeLa whole cell extract. Detection antibody: Monoclonal Anti-WASH1 Clone: WASH1-27 (
Cat. No. SAB4200552).
Lanes1. 5 μg antibody
2. 10 μg antibody
3. Negative control: 10 μg non-relevant antibody