ImmunofluorescencePredominant nuclear localization of TNPO2 in HEK-293T cells over-expressing human TNPO2. Cells were stained with Rabbit Anti-TNPO2 (C-terminal) (
Cat. No. SAB4200031) followed by Goat Anti-Rabbit, Cy3 conjugated. The cells were counterstained with DAPI (blue) to stain nuclei (×40 magnification).
ImmunoprecipitationRabbit Anti-TNPO2 (N-terminal) (
Cat. No. SAB4200031) and Rabbit Anti-TNPO2 (C-terminal) (
Cat. No. SAB4200032) were used to immunoprecipitate human TNPO2 from lysate of HEK-293T cells over-expressing human TNPO2.
Lanes1. IP antibody: 10 μg Anti-TNPO2 (N-terminal) (
Cat. No. SAB4200032)
2. IP antibody: 10 μg Anti-TNPO2 (C-terminal) (
Cat. No. SAB4200031)
3. Negative control: Anti-TNPO2 (N-terminal) IP without cell lysate
4. Negative control: Anti-TNPO2 (C-terminal) IP without cell lysate
5. Negative control: Anti-TNPO2 (N-terminal) IP without IP antibody
6. Negative control: Anti-TNPO2 (C-terminal) IP without IP antibody
7. Immunoblotting positive control: total HEK-293T cells over-expressing human TNPO2 blot with Anti-TNPO2 (N-terminal) (
Cat. No. SAB4200032)
8. Immunoblotting positive control: total HEK-293T cells over-expressing human TNPO2 blot with Anti-TNPO2 (C-terminal) (