Imprint® Anti-TIP5 antibody produced in rabbit

Name	Imprint® Anti-TIP5 antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	SAB4800013
Prices	$412.50
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal
Applications	ChIP WB
Species Reactivities	Human, Mouse
Description	Rabbit Polyclonal
Gene	BAZ2A
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunoblotting</B><BR/>Anti-TIP5: <B>Cat. No. SAB4800013</B>: Western blot analysis of nuclear extract, 150 μg, from either NIH3T3 or HeLa cells with the Anti-TIP5 antibody diluted 1:1,000 in PBS containing 5% milk powder and 0.1% Tween-20. The position of the protein of interest is indicated on the right; the marker, kDa, is shown on the left.

Immunoblotting
Anti-TIP5: Cat. No. SAB4800013: Western blot analysis of nuclear extract, 150 μg, from either NIH3T3 or HeLa cells with the Anti-TIP5 antibody diluted 1:1,000 in PBS containing 5% milk powder and 0.1% Tween-20. The position of the protein of interest is indicated on the right; the marker, kDa, is shown on the left.

<B>ChIP Results Chart</B><BR/>Anti-TIP5: <B>Cat. No. SAB4800013</B>: ChIP assays were performed using the Anti-TIP5 antibody. Chromatin from HEK293T and NIH3T3 cells was formaldehyde cross-linked and sheared to yield fragments with an average length of 200 to 400 bp. ChIP was performed overnight at 4°C with 100 μg sheared chromatin and either 5 μl of the TIP5 antibody or 5 μl IgG, which was used as a negative IP control. The IP minutesd DNA was analyzed by qPCR with primer sets for the promoter and the coding region of the 28s ribosomal RNA gene. The figure shows the recovery by the TIP5 antibody and by IgG, set to 1, normalised to the input DNA. These results show that, both in HEK293T and in NIH3T3 cells, TIP5 is associated with the promoter, but not with the coding region of the 28srRNA gene.

ChIP Results Chart
Anti-TIP5: Cat. No. SAB4800013: ChIP assays were performed using the Anti-TIP5 antibody. Chromatin from HEK293T and NIH3T3 cells was formaldehyde cross-linked and sheared to yield fragments with an average length of 200 to 400 bp. ChIP was performed overnight at 4°C with 100 μg sheared chromatin and either 5 μl of the TIP5 antibody or 5 μl IgG, which was used as a negative IP control. The IP minutesd DNA was analyzed by qPCR with primer sets for the promoter and the coding region of the 28s ribosomal RNA gene. The figure shows the recovery by the TIP5 antibody and by IgG, set to 1, normalised to the input DNA. These results show that, both in HEK293T and in NIH3T3 cells, TIP5 is associated with the promoter, but not with the coding region of the 28srRNA gene.