BAZ2A

Gene Symbol BAZ2A
Entrez Gene 11176
Alt Symbol TIP5, WALp3
Species Human
Gene Type protein-coding
Description bromodomain adjacent to zinc finger domain, 2A
Other Description TTF-I interacting peptide 5|TTF-I-interacting protein 5|bromodomain adjacent to zinc finger domain protein 2A|transcription termination factor I-interacting protein 5
Swissprots Q68DI8 B3KN66 O00536 Q96H26 O15030 Q9UIF9
Accessions EAW96949 EAW96950 EAW96951 Q9UIF9 AB002312 BAA20773 AF000422 AAB60864 AK023830 BAG51228 AK023842 AK127775 AK303327 BAH13943 BC008965 AAH08965 BC065914 BC152739 AAI52740 CD686304 CR749379 CAH18232 DB215236 XM_011537797 XP_011536099 XM_011537798 XP_011536100 XM_011537799 XP_011536101 XM_011537800 XP_011536102 XM_011537801 XP_011536103 XM_011537802 XP_011536104 XM_011537803 XP_011536105 XM_011537804 XP_011536106 XM_011537805 XP_011536107 XM_011537806 XP_011536108 XM_011537807 XP_011536109 XM_011537808 XP_011536110 XM_011537809 XP_011536111 NM_001300905 NP_001287834 NM_013449 NP_038477
Function Essential component of the NoRC (nucleolar remodeling complex) complex, a complex that mediates silencing of a fraction of rDNA by recruiting histone-modifying enzymes and DNA methyltransferases, leading to heterochromatin formation and transcriptional silencing. In the complex, it plays a central role by being recruited to rDNA and by targeting chromatin modifying enzymes such as HDAC1, leading to repress RNA polymerase I transcription. Recruited to rDNA via its interaction with TTF1 and its ability to recognize and bind histone H4 acetylated on 'Lys- 16' (H4K16ac), leading to deacetylation of H4K5ac, H4K8ac, H4K12ac but not H4K16ac. Specifically binds pRNAs, 150-250 nucleotide RNAs that are complementary in sequence to the rDNA promoter; pRNA- binding is required for heterochromatin formation and rDNA silencing (By similarity). {ECO:0000250}.
Subcellular Location Nucleus, nucleolus {ECO:0000250}. Note=Colocalizes with the basal RNA polymerase I transcription factor UBF in the nucleolus. {ECO:0000250}.
Tissue Specificity Expressed at moderate levels in most tissues analyzed, including heart, brain, placenta, lung, skeletal muscle, kidney and pancreas.

Anti-BAZ2A antibody - ab73303 from Abcam

Prices $359.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Anti-BAZ2A antibody (ab73303) at 1 µg/ml + Placenta (Human) Tissue Lysate - adult normal tissue (ab29745) at 10 µgSecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutionPerformed under reducing conditions.

Anti-BAZ2A antibody - ab135989 from Abcam

Host Rabbit
Clonality Polyclonal
Anti-BAZ2A antibody (ab135989) at 1/500 dilution + HeLa cell lysate at 30 µg

Anti-BAZ2A antibody - ab168529 from Abcam

Prices $370.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal
All lanes : Anti-BAZ2A antibody (ab168529) at 1 µg/mlLane 1 : BAZ2A transfected 293T lysateLane 2 : Non-transfected 293T lysateLysates/proteins at 15 µl per lane.

Anti-BAZ2A / TIP5 Antibody (aa1244-1293) - LS-C200177 from LifeSpan Bioscience

Prices $315.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal
Western blot of the lysates from HeLa cells using BAZ2A antibody.

BAZ2A Antibody - NBP1-81515 from Novus Biologicals

Prices $419.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
Western Blot: BAZ2A Antibody [NBP1-81515] - Lane 1: Marker [kDa] 220, 112, 84, 47, 32, 26, 17<br/>Lane 2: Human cell line RT-4<br/>Lane 3: Human cell line U-251MG sp<br/>Lane 4: Human plasma (IgG/HSA depleted)<br/>Lane 5: Human liver tissue<br/>Lane 6: Human tonsil tissue

BAZ2A Antibody - H00011176-B01P-50ug from Novus Biologicals

Prices $359.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-BAZ2A antibody produced in mouse - SAB1412799 from Sigma-Aldrich

Prices $347.00
Sizes 50 µg
Host Mouse
<B>Immunoblotting</B><BR/>Anti-BAZ2A:<B>Cat. No. SAB1412799</B>: Antibody concentration: 0.1–5 μg/mL. Immunofluorescence band of ~209.6 kDa using BAZ2A transfected 293T lysate.<BR/><B>Lane</B><BR/>1. Transfected 293T lysate.<BR/>2. Non-transfected 293T lysate.

Imprint® Anti-TIP5 antibody produced in rabbit - SAB4800013 from Sigma-Aldrich

Prices $412.50
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<B>Immunoblotting</B><BR/>Anti-TIP5: <B>Cat. No. SAB4800013</B>: Western blot analysis of nuclear extract, 150 μg, from either NIH3T3 or HeLa cells with the Anti-TIP5 antibody diluted 1:1,000 in PBS containing 5% milk powder and 0.1% Tween-20. The position of the protein of interest is indicated on the right; the marker, kDa, is shown on the left.

BAZ2A Antibody (OAAF03395) - OAAF03395 from Aviva Systems Biology

Prices $289.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

BAZ2A Antibody (OAAF06145) - OAAF06145 from Aviva Systems Biology

Prices $289.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

BAZ2A antibody - 70R-35806 from Fitzgerald

Prices $275.00
Sizes 100 µg
Host Rabbit
Clonality Polyclonal

BAZ2A Antibody - abx014282 from Abbexa

Host Rabbit
Clonality Polyclonal

BAZ2A antibody - H00011176-B01P from Acris Antibodies

Prices $360.00
Sizes 50 µg
Host Mouse
Clonality Polyclonal

Anti-Tip5 antibody - STJ96028 from St John's Laboratory Ltd

Host Rabbit
Clonality Polyclonal

BAZ2A Polyclonal Antibody - PA006224-R12-2537 from Syd Labs

Prices $150.00, $250.00
Sizes 50 µg, 100 µg
Host Rabbit
Clonality Polyclonal

BAZ2A Antibody - E11-14806C from Enogene

Prices $225.00
Sizes 100 μg
Host Rabbit

BAZ2A antibody (pAb) - 61707 from ActiveMotif

Prices $95.00, $410.00
Sizes 10 µl, 100 µl
Host Rabbit
Clonality Polyclonal
BAZ2A antibody (pAb) tested by Western blot. BAZ2A antibody detection by Western blot. The analysis was performed using 40 µg of HeLa nuclear extract and BAZ2A at a 1: 500 dilution.

BAZ2A Antibody - C14806 from Assay Biotechnology

Prices $175.00, $275.00
Sizes 50 µl, 100 µl
Host Rabbit
Clonality Polyclonal

BAZ2A Antibody - R12-2537 from Assay Biotechnology

Prices $175.00, $275.00
Sizes 50 µl, 100 µl
Host Rabbit
Clonality Polyclonal
Western blot analysis of the lysates from HeLa cells using BAZ2A antibody.

Anti-BAZ2A (aa 1-1905) polyclonal antibody - DPABH-07234 from Creative Diagnostics

Host Mouse
Clonality Polyclonal

Rabbit Anti-Human BAZ2A Polyclonal Antibody - DPABH-26578 from Creative Diagnostics

Host Rabbit
Clonality Polyclonal

Anti-BAZ2A monoclonal antibody - DCABH-14685 from Creative Diagnostics

Host Rabbit
Clonality Monoclonal

TIP5 Antibody - 100-401-V86 from Rockland Immunochemicals Inc

Prices $319.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal

TIP5 polyclonal antibody - Classic - C15310090 (CS-090-100) from Diagenode

Prices $295.00
Sizes 100 µl
Host Rabbit
Clonality Polyclonal
<strong> Figure 1. ChIP results obtained with the Diagenode antibody directed against TIP5 </strong><br />ChIP assays were performed using the Diagenode antibody against TIP5 (cat. No. CS-090-100). Chromatin from HEK293T and NIH3T3 cells was formaldehyde cross-linked and sheared with the Bioruptor (Diagenode) to yield fragments with an average length of 200 to 400 bp. ChIP was performed overnight at 4°C with 100 μg sheared chromatin and either 5 μl of the TIP5 antibody or 5 μl IgG which was used as negative IP control. The IP’d DNA was analysed by qPCR with primer sets for the promoter and the coding region of the 28s ribosomal RNA gene. Figure 1 shows the recovery by the TIP5 antibody and by IgG (set to 1), normalised to the input DNA. These results show that, both in HEK293T and in NIH3T3 cells, TIP5 is associated with the promoter, but not with the coding region of the 28srRNA gene.

BAZ2A Antibody - CSB-PA437543 from Cusabio

Host Rabbit
Clonality Polyclonal
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