ImmunofluorescenceMevastatin-treated HepG2 cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton X-100. Fixed cells were stained with 1 μg/mL Rabbit Anti-HMGCR (
Cat. No. SAB4200528). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
ImmunoblottingWhole extract of Mevastatin-treated HepG2 cells was separated on SDS-PAGE and probed with Rabbit Anti-HMGCR (
Cat. No. SAB4200528). The antibody was developed using Goat Anti-Rabbit IgG-peroxidase (
Cat. No. A0545) and a chemiluminescent substrate.
Lanes1. 1.5 μg/mL
2. 1.5 μg/mL + HMGCR immunizing peptide