ImmunofluorescenceAnti-APLP1 (556-570):
Cat. No. A4360: Immunofluorescence of HUVEC cells using APLP1 (556-570), Cat. No. A4360 (red) at a 1:1600 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com
ImmunoblottingLysate of HEK-293T cells overexpressing human APLP1 was separated on SDS-PAGE, blotted with Anti-APLP1 (556-570) (
Cat. No. A4360) and developed using Goat Anti-Rabbit IgG-Peroxidase (
Cat. No. A0545) and a chemiluminescent substrate.
Lanes1. Antibody dilution 1:250
2. Negative control (only secondary antibody)
3. Anti-FLAG antibody (
Cat. No. F7425), working concentration 2 μg/mL
ChartAnti-APLP1 (556-570):
Cat. No. A4360: Intensity analysis of Anti-APLP1 (556-570) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.