ImmunofluorescenceA549 cells were fixed and permeabilized with cold methanol followed by aceton. Fixed cells were stained with Mouse Anti- HIBCH Clone: HIBCH-B64 (
Cat. No. SAB4100120) diluted to 1:20. The antibody was developed using Goat Anti-Mouse IgG, Cy3 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
ImmunoblottingLysate of HEK-293T cells expressing FLAG
®-tagged human HIBCH was separated on SDS-PAGE, probed with Mouse Anti-HIBCH Clone: HIBCH-B64 (
Cat. No. SAB4100120). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (
Cat. No. A2304) and a chemiluminescent substrate.
Lanes1. Antibody dilution 1:1,000
2. Antibody dilution 1:2,000
3. Negative control
4. Positive control Anti-FLAG
®
ImmunoblottingA549 cell extract was separated on SDS-PAGE and probed with Mouse Anti-HIBCH Clone: HIBCH-B64 (
Cat. No. SAB4100120). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (
Cat. No. A2304) and a chemiluminescent substrate.
Lanes1. Antibody dilution 1:100
2. Negative control