ImmunofluorescenceU87 cells were fixed and permeabilized with 4% Paraformaldehyde and 0.5% Triton X-100. Fixed cells were stained with Mouse Anti-LIN7B Clone: LIN7B-B68 (
Cat. No. SAB4100086) diluted to 1:50. The antibody was developed using Goat Anti-Mouse IgG, Atto 488 conjugate. Cells were counterstained with DAPI (blue) to stain nuclei.
ImmunoblottingLysate of HEK-293T cells expressing FLAG
®-tagged human LIN7B was separated on SDS-PAGE, probed with Mouse Anti-LIN7B Clone: LIN7B-B68 (
Cat. No. SAB4100086). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (
Cat. No. A2304) and a chemiluminescent substrate.
Lanes1. Antibody dilution 1:2,000
2. Negative control
3. Positive control Anti-FLAG
®
ImmunoblottingU87 cell extract was separated on SDS-PAGE and probed with Mouse Anti-LIN7B Clone: LIN7B-B68 (
Cat. No. SAB4100086). The antibody was developed using Goat Anti-Mouse IgG-Peroxidase (
Cat. No. A2304) and a chemiluminescent substrate.
Lanes1. Antibody dilution 1:500
2. Antibody dilution 1:1,000
3. Negative control