Anti-DGKG (17-30) antibody produced in rabbit

Name	Anti-DGKG (17-30) antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	D5070
Prices	$328.50
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal
Applications	WB
Species Reactivities	Human
Antigen	synthetic peptide corresponding to amino acids 17-30 of human DGKG
Description	Rabbit Polyclonal
Gene	DGKG
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunofluorescence</B><BR/>Anti-DGKG (17-30): <B>Cat. No. D5070</B>: Immunofluorescence of HUVEC cells using DGKG (17-30), Cat. No. D5070 (red) at a 1:50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Immunofluorescence
Anti-DGKG (17-30): Cat. No. D5070: Immunofluorescence of HUVEC cells using DGKG (17-30), Cat. No. D5070 (red) at a 1:50 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images may have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human DGKG was separated on SDS-PAGE, blotted with Anti-DGKG (17-30) (<B>Cat. No. D5070</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. Antibody dilution 1:500 <BR/>2. Antibody dilution 1:500 + DGKG immunizing peptide (human, 17-30)

Immunoblotting
Lysate of HEK-293T cells overexpressing human DGKG was separated on SDS-PAGE, blotted with Anti-DGKG (17-30) (Cat. No. D5070) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. Antibody dilution 1:500
2. Antibody dilution 1:500 + DGKG immunizing peptide (human, 17-30)

<B>Chart</B><BR/>Anti-DGKG (17-30): <B>Cat. No. D5070</B>: Intensity analysis of Anti-DGKG (17-30) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.

Chart
Anti-DGKG (17-30): Cat. No. D5070: Intensity analysis of Anti-DGKG (17-30) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.

Product References

Signaling roles of diacylglycerol kinases. - Signaling roles of diacylglycerol kinases.

Topham MK. J Cell Biochem. 2006 Feb 15;97(3):474-84.

Nuclear transportation of diacylglycerol kinase gamma and its possible function - Nuclear transportation of diacylglycerol kinase gamma and its possible function

Matsubara T, Shirai Y, Miyasaka K, Murakami T, Yamaguchi Y, Ueyama T, Kai M, Sakane F, Kanoh H, Hashimoto T, Kamada S, Kikkawa U, Saito N. J Biol Chem. 2006 Mar 10;281(10):6152-64. Epub 2005 Dec 30.

Diacylglycerol kinase gamma is a novel anionic phospholipid binding protein with - Diacylglycerol kinase gamma is a novel anionic phospholipid binding protein with

Takeshita E, Kume A, Maeda Y, Sakai H, Sakane F. Biochem Biophys Res Commun. 2014 Feb 21;444(4):617-21. doi: