Anti-Importin 5 (N-terminal) antibody produced in rabbit
| Name | Anti-Importin 5 (N-terminal) antibody produced in rabbit |
|---|---|
| Supplier | Sigma-Aldrich |
| Catalog | SAB4200178 |
| Prices | $322.00 |
| Sizes | 200 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Applications | IP ICC/IF WB |
| Species Reactivities | Human |
| Antigen | synthetic peptide corresponding to amino acids 49-62 of human Importin 5 conjugated to KLH |
| Description | Rabbit Polyclonal |
| Gene | IPO5 |
| Conjugate | unconjugated |
| Supplier Page | Shop |
Product images
Immunofluorescence HEK-293T cells expressing human Importin 5 were fixed and stained with 5 μg/mL Rabbit Anti-Importin 5 (N-terminal) (Cat. No. SAB4200178) followed by Goat Anti-Rabbit, Cy3 conjugate and counterstained with DAPI (blue) to stain nuclei (×40 magnification).
ImmunoblottingLysate of JURKAT cells was separated on SDS-PAGE and probed with Rabbit Anti-Importin 5 (N-terminal) (Cat. No. SAB4200178). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescence substrate.
Lanes
1. Antibody 4 μg/mL
2. Antibody 2 μg/mL
3. Antibody 1 μg/mL
ImmunoprecipitationRabbit Anti-Importin 5 (Cat. No. SAB4200178) was used to immunoprecipitate human Importin 5 from HEK-293T cell lysate. Detection antibody: Rabbit Anti Importin 5 (C-terminal) (Cat. No. SAB4200179).
Lanes
1. IP antibody:10 μg
2. IP antibody: 5 μg
3. IP antibody: 2.5 μg
4. Negative control: Without IP antibody
5. Negative control: IP antibody without cell lysate
6. Immunoblotting positive control: HEK-293Tcell lysate