Anti-Rab8 antibody produced in rabbit
| Name | Anti-Rab8 antibody produced in rabbit |
|---|---|
| Supplier | Sigma-Aldrich |
| Catalog | R5530 |
| Prices | $346.00 |
| Sizes | 200 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Applications | ICC/IF WB |
| Species Reactivities | Rat, Human, Mouse |
| Antigen | synthetic peptide corresponding to amino acid residues 170-190 of human RAB8A, with an added cysteine, conjugated to KLH |
| Description | Rabbit Polyclonal |
| Gene | RAB8A |
| Conjugate | unconjugated |
| Supplier Page | Shop |
Product images
Immunofluorescence3T3 cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.4% saponin. Fixed cells were stained with 1:100 Rabbit Anti-Rab8 (Cat. No. R5530). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate.
ImmunofluorescenceNRK cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.4% saponin. Fixed cells were stained with 1:100 Rabbit Anti-Rab8 (Cat. No. R5530). The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate.
ImmunoblottingWhole extract of human Jurkat cells was separated on SDS-PAGE and probed with Rabbit Anti-Rab8 (Cat. No. R5530). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. 1:250 antibody
2. 1:250 antibody + Rab8 immunizing peptide (human, 170-190)
3. 1:500 antibody
4. 1:500 antibody + Rab8 immunizing peptide (human, 170-190)