ImmunoblottingLysates of MDA-MB-231 (lanes 1-2), PC3 (lanes 3-4) and A-549 (lanes 5-6) cells were separated on SDS-PAGE, blotted with Anti-SEH1L (86-100) (
Cat. No.SAB1102604) and developed using Goat Anti-Rabbit IgG-Peroxidase (
Cat. No. A0545) and a chemiluminescent substrate.
Lanes1. Antibody dilution 1:250
2. Antibody dilution 1:250 + SEH1L immunizing peptide (human, 86-100)
3. Antibody dilution 1:250
4. Antibody dilution 1:250 + SEH1L immunizing peptide (human, 86-100)
5. Antibody dilution 1:250
6. Antibody dilution 1:250 + SEH1L immunizing peptide (human, 86-100)
ImmunoblottingLysate of HEK-293T cells overexpressing human SEH1L (86-100) was separated on SDS-PAGE, blotted with Anti-SEH1L (86-100) (
Cat. No. SAB1102604) and developed using Goat Anti-Rabbit IgG-Peroxidase (
Cat. No. A0545) and a chemiluminescent substrate.
Lanes1. Antibody dilution 1:250