ImmunoprecipitationRabbit Anti-VPS4A (
Cat. No. SAB4200022) was used to immunoprecipitate VPS4A from NRK whole cell extract. Detection antibody: Rabbit Anti-VPS4 (
Cat. No. SAB4200025) followed by Monoclonal Anti-Rabbit IgG native-peroxidase (
Cat. No. R3155) and a chemiluminescent substrate.
Lanes1. 10 μg antibody
2. 5 μg antibody
3. Negative control: 5 μg nonrelevant antibody
ImmunoblottingWhole extract of HEK-293T cells overexpressing human VPS4A was separated on SDS-PAGE and probed with Rabbit Anti-VPS4A (
Cat. No. SAB4200022). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (
Cat. No. A0545) and a chemiluminescent substrate.
Lanes1. 1 μg/mL antibody
2. Negative control: without first antibody