Anti-KIAA1033 (N-terminal) antibody produced in rabbit

Name	Anti-KIAA1033 (N-terminal) antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	SAB4200513
Prices	$337.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal
Applications	IP ICC/IF WB
Species Reactivities	Mouse, Rat, Human
Antigen	synthetic peptide corresponding to a sequence at the N-terminal region of human KIAA1033, conjugated to KLH
Description	Rabbit Polyclonal
Gene	KIAA1033
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunofluorescence</B><BR/>HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton<SUP>™</SUP> X-100. Fixed cells were stained with 10 μg/mL Rabbit Anti-KIAA1033 (N-Terminal) (<B>Cat. No. SAB4200513</B>). The antibody was developed using Goat Anti-Rabbit IgG, Atto488 conjugate (<B>Cat. No. 18772</B>). Cells were counterstained with DAPI (blue) to stain nuclei.

Immunofluorescence
HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton^™ X-100. Fixed cells were stained with 10 μg/mL Rabbit Anti-KIAA1033 (N-Terminal) (Cat. No. SAB4200513). The antibody was developed using Goat Anti-Rabbit IgG, Atto488 conjugate (Cat. No. 18772). Cells were counterstained with DAPI (blue) to stain nuclei.

<B>Immunoblotting</B><BR/>Cell line lysates were separated on SDS-PAGE and probed with 4 μg/mL Rabbit Anti-KIAA1033 (N-Terminal) (<B>Cat. No. SAB4200513</B>). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate.<BR/><B>Lanes</B><BR/>1. HeLa<BR/>2. RAW264<BR/>3. A9<BR/>4. JURKAT<BR/>5. A20<BR/>6. A10<BR/>7. DAUDI<BR/>8. AT3-B1

Immunoblotting
Cell line lysates were separated on SDS-PAGE and probed with 4 μg/mL Rabbit Anti-KIAA1033 (N-Terminal) (Cat. No. SAB4200513). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. HeLa
2. RAW264
3. A9
4. JURKAT
5. A20
6. A10
7. DAUDI
8. AT3-B1

<B>Immunoprecipitation</B><BR/>Rabbit Anti-KIAA1033 (N-Terminal) (<B>Cat. No. SAB4200513</B>) was used to immunoprecipitate KIAA1033 from HeLa whole cell extract. Detection antibody: Rabbit Anti-KIAA1033 (<B>Cat. No. SAB4200512</B>).<BR/><B>Lanes</B><BR/>1. Negative control: without 1<SUP>st</SUP> antibody<BR/>2. 20 μg antibody<BR/>3. 10 μg antibody

Immunoprecipitation
Rabbit Anti-KIAA1033 (N-Terminal) (Cat. No. SAB4200513) was used to immunoprecipitate KIAA1033 from HeLa whole cell extract. Detection antibody: Rabbit Anti-KIAA1033 (Cat. No. SAB4200512).
Lanes
1. Negative control: without 1^st antibody
2. 20 μg antibody
3. 10 μg antibody