KIAA1033

Gene Symbol	KIAA1033
Entrez Gene	23325
Alt Symbol	MRT43, SWIP
Species	Human
Gene Type	protein-coding
Description	KIAA1033
Other Description	WASH complex subunit 7\|strumpellin and WASH-interacting protein
Swissprots	Q2M389 Q2NL83 Q8N5W7 Q9UPW7 Q9NVD6 Q8IW61
Accessions	EAW97758 EAW97759 EAW97760 EAW97761 Q2M389 AB028956 BAA82985 AF075010 AI753633 AK001657 BAA91816 AK001728 AK023146 AK026950 AK027218 AL137753 BC012556 BC031358 AAH31358 BC040936 AAH40936 BC104992 AAI04993 BC104994 AAI04995 BC110850 AAI10851 BC143373 AAI43374 XM_005268746 XP_005268803 XM_011538073 XP_011536375 XM_011538074 XP_011536376 XM_011538075 XP_011536377 XM_011538076 XP_011536378 XM_011538077 XP_011536379 XR_944511 NM_001293640 NP_001280569 NM_015275 NP_056090
Function	Acts at least in part as component of the WASH core complex whose assembly at the surface of endosomes sems to inhibit WASH nucleation-promoting factor (NPF) activity in recruiting and activating the Arp2/3 complex to induce actin polymerization, and which is involved in the regulation of the fission of tubules that serve as transport intermediates during endosome sorting (PubMed:19922875, PubMed:20498093). {ECO:0000250\|UniProtKB:Q3UMB9, ECO:0000303\|PubMed:21498477, ECO:0000305\|PubMed:19922875, ECO:0000305\|PubMed:20498093}.
Subcellular Location	Early endosome {ECO:0000269\|PubMed:23676666}.

KIAA1033 (N-20) - sc-139583 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	100 µg/ml
Host	Rabbit
Clonality	Polyclonal

KIAA1033 (S-19) - sc-139584 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	100 µg/ml
Host	Rabbit
Clonality	Polyclonal

KIAA1033 (S-19): sc-139584. Western blot analysis of KIAA1033 expression in PC-12 whole cell lysate.

KIAA1033 (P-19) - sc-139585 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	100 µg/ml
Host	Rabbit
Clonality	Polyclonal

KIAA1033 (P-19): sc-139585. Western blot analysis of KIAA1033 expression in K-562 whole cell lysate (A) and mouse heart (B) and mouse brain (C) tissue extracts.

Anti-WASH complex subunit 7 antibody - ab111109 from Abcam

Prices	$370.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

Anti-KIAA1033 Antibody (Internal) - LS-C145164 from LifeSpan Bioscience

Prices	$365.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Anti-KIAA1033 antibody produced in rabbit - HPA045666 from Sigma-Aldrich

Prices	$429.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunohistochemistry</B><BR/>Anti-KIAA1033: <B>Cat. No. HPA045666</B>: Immunohistochemical staining of Human colon shows strong nuclear positivity in glandular cells.

Anti-KIAA1033 antibody produced in rabbit - HPA046737 from Sigma-Aldrich

Prices	$429.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunohistochemistry</B><BR/>Anti-KIAA1033: <B>Cat. No. HPA046737</B>: Immunohistochemical staining of Human stomach, lower shows strong cytoplasmic and membranous positivity in glandular cells.

Anti-KIAA1033 antibody produced in rabbit - SAB4200512 from Sigma-Aldrich

Prices	$337.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Cell line lysates were separated on SDS-PAGE and probed with 4 μg/mL Rabbit Anti-KIAA1033 (<B>Cat. No. SAB4200512</B>). The antibody was developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate.<BR/><B>Lanes</B><BR/>1. HeLa <BR/>2. RAW264<BR/>3. A9<BR/>4. JURKAT<BR/>5. A20<BR/>6. A10<BR/>7. DAUDI<BR/>8. AT3-B1

Anti-KIAA1033 (N-terminal) antibody produced in rabbit - SAB4200513 from Sigma-Aldrich

Prices	$337.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunofluorescence</B><BR/>HeLa cells were fixed and permeabilized with 4% paraformaldehyde followed by 0.5% Triton<SUP>™</SUP> X-100. Fixed cells were stained with 10 μg/mL Rabbit Anti-KIAA1033 (N-Terminal) (<B>Cat. No. SAB4200513</B>). The antibody was developed using Goat Anti-Rabbit IgG, Atto488 conjugate (<B>Cat. No. 18772</B>). Cells were counterstained with DAPI (blue) to stain nuclei.

KIAA1033 antibody - 51101-1-AP from Acris Antibodies

Prices	$270.00
Sizes	150 µl
Host	Rabbit
Clonality	Polyclonal

HeLa cells were subjected to SDS PAGE followed by western blot with 51101-1-AP( KIAA1033 Antibody) at dilution of 1:300

WASH complex subunit-7 antibody - AP55395SU-N from Acris Antibodies

Prices	$460.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

KIAA1033 antibody - 51101-1-AP from Proteintech Group

Prices	$279.00
Sizes	con: 22 μg/150μl
Host	Rabbit
Clonality	Polyclonal

KIAA0999, Polyclonal Antibody - MBS6001296 from MyBioSource

Prices	$550.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

KIAA1033, Polyclonal Antibody - MBS2521831 from MyBioSource

Prices	$195.00, $270.00, $435.00
Sizes	60 µl, 120 µl, 200 µl
Host	Rabbit
Clonality	Polyclonal

KIAA1033 Antibody - EPP14648 from Elabscience

Prices	$296.00, $485.00
Sizes	120 μl, 200 μl
Host	Rabbit
Clonality	Polyclonal

Anti-KIAA1033 (internal region) polyclonal antibody - CPBT-49784RH from Creative Diagnostics

Host	Rabbit
Clonality	Polyclonal

Anti-WASH complex subunit 7 (SWIP) Antibody - ABT70 from EMD Millipore

Prices	$319.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

WASH complex subunit-7 (KIAA1033) antibody - pab0732 from Covalab

Host	Rabbit
Clonality	Polyclonal

WASH complex subunit 7 antibody - AP56118PU-N from Acris Antibodies

Prices	$380.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Immunoprecipitation/Western Blotting Analysis: Representative lot data. HeLa immunoprecipitation with rabbit normal IgG (lane 1) and AP56118PU-N Anti-WASH complex subunit 7 (lane 2) was probed with Anti-WASH complex subunit 7 (SWIP) (5 µg/mL). Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and chemiluminescence detection system. Arrow indicates WASH complex subunit 7 (~135 kDa). IgG heavy chain may be observed at approximately 50 kDa.

KIAA1033/WASH complex subunit 7 Antibody - A304-918A from Bethyl Labs

Prices	$99.00, $339.00
Sizes	10 µl (1 mg/ml), 100 µl (1 mg/ml)
Host	Rabbit
Clonality	Polyclonal

KIAA1033/WASH complex subunit 7 Antibody - A304-919A from Bethyl Labs

Prices	$99.00, $229.00, $339.00
Sizes	20 µl (2 blots), 100 µl (10 blots), 100 µl (1 mg/ml)
Host	Rabbit
Clonality	Polyclonal

KIAA1033/WASH complex subunit 7 Antibody AbVantage™ Pack - A311-688A from Bethyl Labs

Prices	$472.00
Sizes	1 pack
Host	Rabbit
Clonality	Polyclonal

KIAA1033 Antibody - NBP2-44291 from Novus Biologicals

Prices	$349.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: KIAA1033 Antibody [NBP2-44291] - Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. NBP2-44291 was used for IP at 6 ug per reaction. KIAA1033 was also immunoprecipitated by NBP2-44290.

KIAA1033 Antibody - NBP2-44290 from Novus Biologicals

Prices	$349.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Immunoprecipitation: KIAA1033 Antibody [NBP2-44290] - Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.NBP2-44290 was used for IP at 6 ug per reaction. KIAA1033 was also immunoprecipitated by NBP2-44291.

Anti-WASH complex subunit 7 (SWIP) Antibody - ABT70 from Sigma-Aldrich

Prices	$319.00
Sizes	abt70
Clonality	Polyclonal

Immunoprecipitation/Western Blotting Analysis: <br />Representative lot data. <br />HeLa immunoprecipitation with rabbit normal IgG (lane 1) and ABT70 Anti-WASH complex subunit 7 (lane 2) was probed with Anti-WASH complex subunit 7 (SWIP) (5 µg/mL). <br />Proteins were visualized using a Donkey Anti-Rabbit IgG secondary antibody conjugated to HRP and chemiluminescence detection system. <br />Arrow indicates WASH complex subunit 7 (~135 kDa). IgG heavy chain may be observed at approximately 50 kDa.

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