Anti-Par6β antibody produced in rabbit

Name	Anti-Par6β antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	B8062
Prices	$57.50, $332.50
Sizes	25 µl, 200 µl
Host	Rabbit
Clonality	Polyclonal
Applications	IP ICC/IF WB
Species Reactivities	Human
Antigen	synthetic peptide corresponding to amino acids 275-289 of human Par6β conjugated to KLH
Description	Rabbit Polyclonal
Gene	PARD6B
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunofluorescence</B><BR/>Nuclear and cell cell contacts localization of PAR6β in MDCK cells. Fixed cells were stained with 2 μg/mL Rabbit Anti-PAR6β (<B>Cat. No. B8062</B>) followed by Goat Anti-Rabbit IgG, Cy3 conjugate and counterstained with DAPI (blue) to stain nuclei (x40 magnification).

Immunofluorescence
Nuclear and cell cell contacts localization of PAR6β in MDCK cells. Fixed cells were stained with 2 μg/mL Rabbit Anti-PAR6β (Cat. No. B8062) followed by Goat Anti-Rabbit IgG, Cy3 conjugate and counterstained with DAPI (blue) to stain nuclei (x40 magnification).

<B>Immunoprecipitation</B><BR/>Rabbit Anti-PAR6β (<B>Cat. No. B8062</B>) was used to immunoprecipitate PAR6β from MDCK cells lysate as indicated below. The detection antibody was developed with Monoclonal Anti-Rabbit IgG (<B>Cat. No. R3155</B>) and a chemiluminescent substrate.<BR/><B>Lanes</B><BR/>1. Immunoblotting positive control – 2 μg/mL Rabbit Anti-PAR6β (<B>Cat. No. B8062</B>).<BR/>2. IP antibody: 5 μg/mL Rabbit Anti-PAR6β (<B>Cat. No. B8062</B>). Detection antibody: Anti-PAR6β (C-Terminal) (<B>Cat. No. B8187</B>)<BR/>3. IP antibody: 2 μg/mL Rabbit Anti-PAR6β (<B>Cat. No. B8062</B>). Detection antibody: Anti-PAR6β (C-Terminal) (<B>Cat. No. B8187</B>)<BR/>4. Negative control – without IP antibody.<BR/>5. Negative control – without cell lysate.

Immunoprecipitation
Rabbit Anti-PAR6β (Cat. No. B8062) was used to immunoprecipitate PAR6β from MDCK cells lysate as indicated below. The detection antibody was developed with Monoclonal Anti-Rabbit IgG (Cat. No. R3155) and a chemiluminescent substrate.
Lanes
1. Immunoblotting positive control – 2 μg/mL Rabbit Anti-PAR6β (Cat. No. B8062).
2. IP antibody: 5 μg/mL Rabbit Anti-PAR6β (Cat. No. B8062). Detection antibody: Anti-PAR6β (C-Terminal) (Cat. No. B8187)
3. IP antibody: 2 μg/mL Rabbit Anti-PAR6β (Cat. No. B8062). Detection antibody: Anti-PAR6β (C-Terminal) (Cat. No. B8187)
4. Negative control – without IP antibody.
5. Negative control – without cell lysate.

Product References

Willin and Par3 cooperatively regulate epithelial apical constriction through - Willin and Par3 cooperatively regulate epithelial apical constriction through

Ishiuchi T, Takeichi M. Nat Cell Biol. 2011 Jun 19;13(7):860-6.

Touchdown PCR for increased specificity and sensitivity in PCR amplification. - Touchdown PCR for increased specificity and sensitivity in PCR amplification.

Korbie DJ, Mattick JS. Nat Protoc. 2008;3(9):1452-6.