ImmunofluorescenceCytoplasmic and membrane localization of tensin 2 in NIH3T3 cells overexpressing human tensin 2. Fixed cells were stained with 1 μg/mL Anti-Tensin 2 (N-terminal) (
Cat. No. SAB4200269), followed by Goat Anti-Rabbit IgG, Cy3 conjugate (×40 magnification). Nuclear staining with DAPI.
ImmunoblottingCell lysates of HEK293-T overexpressing human tensin 2 (lanes 1,3), HEK293-T non-transfected (lane 2), were separated on SDS-PAGE, blotted with Anti-Tensin 2 (N-terminal) (
Cat. No. SAB4200269), and developed using Goat Anti-Rabbit IgG-Peroxidase (
Cat. No. A0545) and a chemiluminescent substrate.
Lanes1. Antibody: 1.5 μg/mL
2. Antibody: 1.5 μg/mL
3. Antibody: 1.5 μg/mL + immunizing tensin 2 peptide