Anti-PSPC1 (C-terminal) antibody produced in rabbit

Name	Anti-PSPC1 (C-terminal) antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	SAB4200067
Prices	$354.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal
Applications	IP ICC/IF WB
Species Reactivities	Human
Antigen	synthetic peptide corresponding to amino acids 376-390 of human PSPC1 conjugated to KLH
Description	Rabbit Polyclonal
Gene	PSPC1
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunofluorescence</B><BR/>Staining of PSPC1 in HEK-293T cells. Cells were fixed and stained with 1.25 μg/mL Rabbit Anti-PSPC1 (C-Terminal) (<B>Cat. No. SAB4200067</B>) followed by Goat Anti-Rabbit, Cy3 conjugate and counterstained with DAPI (blue) to stain nuclei (×40 magnification).

Immunofluorescence
Staining of PSPC1 in HEK-293T cells. Cells were fixed and stained with 1.25 μg/mL Rabbit Anti-PSPC1 (C-Terminal) (Cat. No. SAB4200067) followed by Goat Anti-Rabbit, Cy3 conjugate and counterstained with DAPI (blue) to stain nuclei (×40 magnification).

<B>Immunoblotting</B><BR/>Lysates of the indicated cells were separated on SDS-PAGE and probed with 2 μg/mL Rabbit Anti-PSPC1 (C-Terminal) (<B>Cat. No. SAB4200067</B>). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (<B>Cat. No. A0545</B>) and a chemiluminescence substrate. <BR/><B>Lanes</B><BR/>1. HeLa; 2. K-562; 3. Raw264; 4. HEK-293T; 5. HEK-293T over-expressing PSPC1

Immunoblotting
Lysates of the indicated cells were separated on SDS-PAGE and probed with 2 μg/mL Rabbit Anti-PSPC1 (C-Terminal) (Cat. No. SAB4200067). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescence substrate.
Lanes
1. HeLa; 2. K-562; 3. Raw264; 4. HEK-293T; 5. HEK-293T over-expressing PSPC1

<B>Immunoblotting</B><BR/>Lysate of HEK-293Tcells was separated on SDS-PAGE and probed with Rabbit Anti-PSPC1 (C-terminal) (<B>Cat. No. SAB4200067</B>). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (<B>Cat. No. A0545</B>) and a chemiluminescence substrate. <BR/><B>Lanes</B><BR/>1. Antibody 2 μg/mL<BR/>2. Antibody 1 μg/ mL<BR/>3. Antibody 0.5 μg/ mL <BR/>4. Antibody 1 μg/mL + 10 μg/mL PSPC1 immunizing peptide

Immunoblotting
Lysate of HEK-293Tcells was separated on SDS-PAGE and probed with Rabbit Anti-PSPC1 (C-terminal) (Cat. No. SAB4200067). The antibody was developed with Goat Anti-Rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminescence substrate.
Lanes
1. Antibody 2 μg/mL
2. Antibody 1 μg/ mL
3. Antibody 0.5 μg/ mL
4. Antibody 1 μg/mL + 10 μg/mL PSPC1 immunizing peptide

<B>Immunoprecipitation</B><BR/>Rabbit Anti-PSPC1 (C-Terminal) (<B>Cat. No. SAB4200067</B>) was used to immunoprecipitate human PSPC1 from lysate of HEK-293T cells. Detection Antibody: Rabbit Anti-PSPC1 (N-Terminal) (<B>Cat. No. SAB4200068</B>).<BR/><B>Lanes</B><BR/>1. IP antibody: 10 μg<BR/>2. IP antibody: 5 μg<BR/>3. IP antibody: 2.5 μg

Immunoprecipitation
Rabbit Anti-PSPC1 (C-Terminal) (Cat. No. SAB4200067) was used to immunoprecipitate human PSPC1 from lysate of HEK-293T cells. Detection Antibody: Rabbit Anti-PSPC1 (N-Terminal) (Cat. No. SAB4200068).
Lanes
1. IP antibody: 10 μg
2. IP antibody: 5 μg
3. IP antibody: 2.5 μg