Anti-HACE1 (376-390) antibody produced in rabbit

Name	Anti-HACE1 (376-390) antibody produced in rabbit
Supplier	Sigma-Aldrich
Catalog	H4540
Prices	$342.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal
Applications	WB
Species Reactivities	Human
Antigen	synthetic peptide corresponding to amino acids 376-390 of human HACE1
Description	Rabbit Polyclonal
Gene	HACE1
Conjugate	unconjugated
Supplier Page	Shop

Product images

<B>Immunofluorescence</B><BR/>Anti-HACE1 (376-390): <B>Cat. No. H4540</B>: Immunofluorescence of HUVEC cells using HACE1 (376-390) (RB),<B>Cat. No. H4540</B> (red) at a 1:100 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Immunofluorescence
Anti-HACE1 (376-390): Cat. No. H4540: Immunofluorescence of HUVEC cells using HACE1 (376-390) (RB),Cat. No. H4540 (red) at a 1:100 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

<B>Immunoblotting</B><BR/>Lysate of HEK-293T cells overexpressing human HACE1 was separated on SDS-PAGE, blotted with Anti-HACE1 (376-390) (<B>Cat. No. H4540</B>) and developed using Goat Anti-Rabbit IgG-Peroxidase (<B>Cat. No. A0545</B>) and a chemiluminescent substrate. <BR/><B>Lanes</B><BR/>1. Antibody dilution 1:500 <BR/>2. Antibody dilution 1:500 + HACE1 immunizing peptide (human, 376-390) <BR/>3. Antibody dilution 1:1,000

Immunoblotting
Lysate of HEK-293T cells overexpressing human HACE1 was separated on SDS-PAGE, blotted with Anti-HACE1 (376-390) (Cat. No. H4540) and developed using Goat Anti-Rabbit IgG-Peroxidase (Cat. No. A0545) and a chemiluminescent substrate.
Lanes
1. Antibody dilution 1:500
2. Antibody dilution 1:500 + HACE1 immunizing peptide (human, 376-390)
3. Antibody dilution 1:1,000

<B>Chart</B><BR/>Anti-HACE1 (376-390): <B>Cat. No. H4540</B>: Intensity analysis of Anti-HACE1 (376-390) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.

Chart
Anti-HACE1 (376-390): Cat. No. H4540: Intensity analysis of Anti-HACE1 (376-390) staining. Cytoplasmic and nuclear intensity values were obtained at 8 different antibody dilutions (1:50 to 1:6,400) as compared with the negative control. Yale HTCB IF procedure used.