HACE1

Gene Symbol	HACE1
Entrez Gene	57531
Alt Symbol	-
Species	Human
Gene Type	protein-coding
Description	HECT domain and ankyrin repeat containing E3 ubiquitin protein ligase 1
Other Description	E3 ubiquitin-protein ligase HACE1
Swissprots	B4DFM6 Q8ND12 Q5VU99 E9PGP0 B3KY89 B4DTQ4 Q5VUA0 A8K6U5 Q9P2M6 B7Z9X6 Q8IYU2
Accessions	CCQ43645 EAW48437 EAW48438 EAW48439 Q8IYU2 AB037741 BAA92558 AJ420474 AK124268 AK131207 BAG54751 AK291760 BAF84449 AK292683 BAF85372 AK294164 BAG57487 AK300314 BAG62066 AK311633 AK316091 BAH14462 AL834202 CAD38890 BC034982 AAH34982 BC038119 BC041919 DA651352 DQ891735 ABM82661 XM_006715528 XP_006715591 XM_006715529 XP_006715592 XM_006715530 XP_006715593 XM_011535989 XP_011534291 XM_011535990 XP_011534292 XM_011535991 XP_011534293 XM_011535992 XP_011534294 XR_942529 XR_942531 NM_020771 NP_065822 NR_104424
Function	E3 ubiquitin-protein ligase involved in Golgi membrane fusion and regulation of small GTPases. Acts as a regulator of Golgi membrane dynamics during the cell cycle: recruited to Golgi membrane by Rab proteins and regulates postmitotic Golgi membrane fusion. Acts by mediating ubiquitination during mitotic Golgi disassembly, ubiquitination serving as a signal for Golgi reassembly later, after cell division. Specifically interacts with GTP-bound RAC1, mediating ubiquitination and subsequent degradation of active RAC1, thereby playing a role in host defense against pathogens. May also act as a transcription regulator via its interaction with RARB. {ECO:0000269\|PubMed:15254018, ECO:0000269\|PubMed:21988917, ECO:0000269\|PubMed:22036506}.
Subcellular Location	Golgi apparatus, Golgi stack membrane. Cytoplasm. Endoplasmic reticulum. Note=A significant portion localizes to the endoplasmic reticulum. Targeted to Golgi membrane via its interaction with Rab proteins.
Tissue Specificity	Expressed in multiple tissues including heart, brain and kidney. {ECO:0000269\|PubMed:15254018}.

HACE1 (T-14) - sc-139143 from Santa Cruz Biotechnology

Prices	$279.00
Sizes	200 µg/ml
Host	Goat
Clonality	Polyclonal

Anti-HACE1 antibody - ab128256 from Abcam

Prices	$372.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-HACE1 antibody [EPR7962] - ab133637 from Abcam

Prices	$379.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR7962

All lanes : Anti-HACE1 antibody [EPR7962] (ab133637) at 1/1000 dilutionLane 1 : 293T cell lysateLane 2 : SH-SY5Y cell lysate at 10 µgSecondaryHRP labelled goat anti-rabbit at 1/2000 dilution

Anti-HACE1 Antibody (aa792-841) - LS-C102017 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

HACE1 antibody LS-C102017 Western blot of HeLa lysate.

Anti-HACE1 Antibody (N-Terminus) - LS-C201445 from LifeSpan Bioscience

Prices	$365.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Western blot of Human OVCAR-3. HACE1 antibody dilution 1.0 ug/ml.

Anti-HACE1 Antibody (Internal, clone EPR7962) - LS-C154466 from LifeSpan Bioscience

Prices	$475.00
Sizes	100 µl
Host	Rabbit
Clonality	Monoclonal
Clone	EPR7962

IHC of paraffin-embedded human astrocytoma tissue using anti-HACE1 antibody.

Anti-HACE1 Antibody (Internal) - LS-C153439 from LifeSpan Bioscience

Prices	$315.00
Sizes	100 µg
Host	Rabbit
Clonality	Polyclonal

Anti-HACE1 Antibody (aa800-900) - LS-C308172 from LifeSpan Bioscience

Prices	$455.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal

Anti-HACE1 Antibody (clone 8D3, Biotin) - LS-C347808 from LifeSpan Bioscience

Prices	$285.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	8D3

Anti-HACE1 Antibody (clone 8D3, FITC) - LS-C347809 from LifeSpan Bioscience

Prices	$295.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	8D3

Anti-HACE1 Antibody (clone 8D3) - LS-C348205 from LifeSpan Bioscience

Prices	$345.00
Sizes	200 µg
Host	Mouse
Clonality	Monoclonal
Clone	8D3

HACE1 Antibody - NBP1-55062 from Novus Biologicals

Prices	$329.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

Western Blot: HACE1 Antibody [NBP1-55062] - Titration: 0.2-1 ug/ml, Positive Control: Hela cell lysate.

HACE1 Antibody (2B3) - H00057531-M04 from Novus Biologicals

Prices	$359.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	2B3

Monoclonal Anti-HACE1 antibody produced in mouse - WH0057531M4 from Sigma-Aldrich

Prices	$347.00
Sizes	100 µg
Host	Mouse
Clonality	Monoclonal
Clone	2B3

Anti-HACE1 antibody produced in rabbit - SAB2101010 from Sigma-Aldrich

Prices	$347.50
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

<B>Immunoblotting</B><BR/>Anti-HACE1: <B>Cat. No.SAB2101010</B>: HACE1 in HeLa cell lysate with HACE1 antibody at 1.0 μg/mL.

Anti-HACE1 (376-390) antibody produced in rabbit - H4540 from Sigma-Aldrich

Prices	$342.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunofluorescence</B><BR/>Anti-HACE1 (376-390): <B>Cat. No. H4540</B>: Immunofluorescence of HUVEC cells using HACE1 (376-390) (RB),<B>Cat. No. H4540</B> (red) at a 1:100 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

Anti-HACE1 (796-810) antibody produced in rabbit - H4665 from Sigma-Aldrich

Prices	$342.00
Sizes	200 µl
Host	Rabbit
Clonality	Polyclonal

<B>Immunofluorescence</B><BR/>Anti-HACE1 (796-810): <B>Cat. No. H4665</B>: Immunofluorescence of HUVEC cells using HACE1 (796-810) (RB),<B>Cat. No. H4665</B> (red) at a 1:100 dilution, taken at 40× magnification and nuclear staining with Hoescht 33342 (blue).Yale HTCB IF procedure used. Images have been adjusted to improve viewing quality. If you would like the original image, please send a request to protocols@sial.com

HACE1 Antibody - N-terminal region (ARP43276_P050) - ARP43276_P050 from Aviva Systems Biology

Prices	$289.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

HACE1 antibody - middle region (ARP43277_P050) - ARP43277_P050 from Aviva Systems Biology

Prices	$289.00
Sizes	100 µl
Host	Rabbit
Clonality	Polyclonal

HACE1 antibody, Internal - GTX46909 from GeneTex

Prices	$299.00
Sizes	50 μg
Host	Rabbit
Clonality	Polyclonal

Anti-HACE1 antibody, Internal used in Western blot. GTX46909

HACE1 antibody - orb100314 from Biorbyt

Prices	$255.00, $340.00
Sizes	100 μg, 200 μg
Host	Rabbit
Clonality	Polyclonal

IHC-P of human colon cancer using HACE1 antibody.

HACE1 antibody - 70R-2804 from Fitzgerald

Prices	$375.00
Sizes	50 µg
Host	Rabbit
Clonality	Polyclonal

Anti -HACE1 (E3 Ubiquitin-protein Ligase HACE1, HECT Domain and Ankyrin Repeat-containingE3 Ubiquitin-protein Ligase 1, KIAA1320) - 127704 from USBiological Life Science

Host	Mouse
Clonality	Monoclonal
Clone	2B3

Anti -HACE1 (E3 Ubiquitin-protein Ligase HACE1, HECT Domain and Ankyrin Repeat-containingE3 Ubiquitin-protein Ligase 1, KIAA1320) (AP) - 127704-AP from USBiological Life Science

Host	Mouse
Clonality	Monoclonal
Clone	2B3

Anti -HACE1 (E3 Ubiquitin-protein Ligase HACE1, HECT Domain and Ankyrin Repeat-containingE3 Ubiquitin-protein Ligase 1, KIAA1320) (Biotin) - 127704-Biotin from USBiological Life Science

Host	Mouse
Clonality	Monoclonal
Clone	2B3

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